BACKGROUND INFORMATION: We characterized previously a cellular protein through its interaction with cellular and viral transcription factors from the bZip family. The corresponding mRNA was detected in a wide range of cell types and the protein was highly expressed in the nucleus of human keratinocytes. On the basis of these observations, we named this protein ubinuclein. RESULTS: Using a specific monoclonal antibody, we have shown in the present study that, although endogenous ubinuclein was mainly nuclear in sparse MDCK (Madin-Darby canine kidney) cells, it was exclusively present in the cell-cell junctions in confluent MDCK cultures or in polarized HT29 cells, where it co-localized with the tight junction marker ZO-1 (zonula occludens 1). In accordance with this, we have shown that ubinuclein interacted with ZO-1 in vitro and in vivo. In cultures of undifferentiated human keratinocytes, ubinuclein was essentially nuclear, but in differentiated cells, in which involucrin and periplakin reside at the apical cell membrane and at the cell-cell junctions, ubinuclein staining was observed at the lateral cell-cell borders. In human skin, ubinuclein appeared as a thread-like pattern between the upper granular cell layer and the cornified cell layer. In mouse epithelia, including bile canaliculi, bronchioli, salivary gland ducts, and oral and olfactory epithelium, ubinuclein co-localized with tight junction markers. Ubinuclein was, however, not present in endothelial cell-cell junctions. In addition, when overexpressed, ubinuclein localized to the nucleus and prevented MDCK cells from entering cytokinesis, resulting in multinucleated giant cells after several cycles of endoreplication. CONCLUSIONS: Ubinuclein mRNA and its corresponding protein are expressed in almost all cell types. Analyses have revealed that in most cells ubinuclein occurred in the nucleoplasm, but in cells forming tight junctions it is recruited to the plaque structure of the zonula occludens. This recruitment appeared to be dependent on cell density. Therefore ubinuclein is a new NACos (nuclear and adhesion complex component) protein.
BACKGROUND INFORMATION: We characterized previously a cellular protein through its interaction with cellular and viral transcription factors from the bZip family. The corresponding mRNA was detected in a wide range of cell types and the protein was highly expressed in the nucleus of human keratinocytes. On the basis of these observations, we named this protein ubinuclein. RESULTS: Using a specific monoclonal antibody, we have shown in the present study that, although endogenous ubinuclein was mainly nuclear in sparse MDCK (Madin-Darby canine kidney) cells, it was exclusively present in the cell-cell junctions in confluent MDCK cultures or in polarized HT29 cells, where it co-localized with the tight junction marker ZO-1 (zonula occludens 1). In accordance with this, we have shown that ubinuclein interacted with ZO-1 in vitro and in vivo. In cultures of undifferentiated human keratinocytes, ubinuclein was essentially nuclear, but in differentiated cells, in which involucrin and periplakin reside at the apical cell membrane and at the cell-cell junctions, ubinuclein staining was observed at the lateral cell-cell borders. In human skin, ubinuclein appeared as a thread-like pattern between the upper granular cell layer and the cornified cell layer. In mouse epithelia, including bile canaliculi, bronchioli, salivary gland ducts, and oral and olfactory epithelium, ubinuclein co-localized with tight junction markers. Ubinuclein was, however, not present in endothelial cell-cell junctions. In addition, when overexpressed, ubinuclein localized to the nucleus and prevented MDCK cells from entering cytokinesis, resulting in multinucleated giant cells after several cycles of endoreplication. CONCLUSIONS: Ubinuclein mRNA and its corresponding protein are expressed in almost all cell types. Analyses have revealed that in most cells ubinuclein occurred in the nucleoplasm, but in cells forming tight junctions it is recruited to the plaque structure of the zonula occludens. This recruitment appeared to be dependent on cell density. Therefore ubinuclein is a new NACos (nuclear and adhesion complex component) protein.
Authors: Yong Tang; Aastha Puri; M Daniel Ricketts; Taranjit Singh Rai; Jason Hoffmann; Elise Hoi; Peter D Adams; David C Schultz; Ronen Marmorstein Journal: Biochemistry Date: 2012-03-16 Impact factor: 3.162
Authors: H C Bauer; A Traweger; J Zweimueller-Mayer; C Lehner; H Tempfer; I Krizbai; I Wilhelm; H Bauer Journal: J Neural Transm (Vienna) Date: 2010-09-24 Impact factor: 3.575
Authors: Alicia M Blessing; Sathya Ganesan; Kimal Rajapakshe; Ying Ying Sung; Lakshmi Reddy Bollu; Yan Shi; Edwin Cheung; Cristian Coarfa; Jeffrey T Chang; Donald P McDonnell; Daniel E Frigo Journal: Mol Endocrinol Date: 2015-08-25
Authors: Guillermo A Orsi; Ahmed Algazeery; Régis E Meyer; Michèle Capri; Laure M Sapey-Triomphe; Béatrice Horard; Henri Gruffat; Pierre Couble; Ounissa Aït-Ahmed; Benjamin Loppin Journal: PLoS Genet Date: 2013-02-07 Impact factor: 5.917