Literature DB >> 18808336

Complexity of Plasmodium falciparum clinical samples from Uganda during short-term culture.

Samuel L Nsobya1, Moses Kiggundu, Moses Joloba, Grant Dorsey, Philip J Rosenthal.   

Abstract

We cultured Plasmodium falciparum parasites from 98 Ugandan children with malaria and determined the complexity of infection (COI) on the basis of msp-2 polymorphisms daily for 9 days. The mean COI decreased during culture from 1.73 to 1.56. New strains appeared after day 0 in 20 cultures. Strains disappeared after day 0 in 56% of 45 cultures that were initially mixed; persisting strains more commonly had wild-type dhfr (C59) and dhps (K540) sequences and mutant pfmdr1 (86Y) sequences. Thus, initial genotypes offer an imperfect representation of clinical COI. Loss of strains in culture may be due to diminished fitness of some drug-resistant strains.

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Year:  2008        PMID： 18808336     DOI： 10.1086/592506

Source DB:  PubMed          Journal:  J Infect Dis        ISSN： 0022-1899            Impact factor:   5.226


  19 in total

1.  Differential association of Plasmodium falciparum Na+/H+ exchanger polymorphism and quinine responses in field- and culture-adapted isolates of Plasmodium falciparum.

Authors:  Stéphane Pelleau; Lionel Bertaux; Sébastien Briolant; Michael T Ferdig; Véronique Sinou; Bruno Pradines; Daniel Parzy; Ronan Jambou
Journal:  Antimicrob Agents Chemother       Date:  2011-09-26       Impact factor: 5.191

2.  Differential prevalence of transporter polymorphisms in symptomatic and asymptomatic falciparum malaria infections in Uganda.

Authors:  Stephen Tukwasibwe; Levi Mugenyi; George W Mbogo; Sheila Nankoberanyi; Catherine Maiteki-Sebuguzi; Moses L Joloba; Samuel L Nsobya; Sarah G Staedke; Philip J Rosenthal
Journal:  J Infect Dis       Date:  2014-01-19       Impact factor: 5.226

3.  Fitness Consequences of Plasmodium falciparum pfmdr1 Polymorphisms Inferred from Ex Vivo Culture of Ugandan Parasites.

Authors:  Edwin Ochong; Patrick K Tumwebaze; Oswald Byaruhanga; Bryan Greenhouse; Philip J Rosenthal
Journal:  Antimicrob Agents Chemother       Date:  2013-06-24       Impact factor: 5.191

Review 4.  Harnessing genomics and genome biology to understand malaria biology.

Authors:  Sarah K Volkman; Daniel E Neafsey; Stephen F Schaffner; Daniel J Park; Dyann F Wirth
Journal:  Nat Rev Genet       Date:  2012-04-12       Impact factor: 53.242

5.  Ex Vivo Drug Susceptibility Testing and Molecular Profiling of Clinical Plasmodium falciparum Isolates from Cambodia from 2008 to 2013 Suggest Emerging Piperaquine Resistance.

Authors:  Suwanna Chaorattanakawee; David L Saunders; Darapiseth Sea; Nitima Chanarat; Kritsanai Yingyuen; Siratchana Sundrakes; Piyaporn Saingam; Nillawan Buathong; Sabaithip Sriwichai; Soklyda Chann; Youry Se; You Yom; Thay Kheng Heng; Nareth Kong; Worachet Kuntawunginn; Kuntida Tangthongchaiwiriya; Christopher Jacob; Shannon Takala-Harrison; Christopher Plowe; Jessica T Lin; Char Meng Chuor; Satharath Prom; Stuart D Tyner; Panita Gosi; Paktiya Teja-Isavadharm; Chanthap Lon; Charlotte A Lanteri
Journal:  Antimicrob Agents Chemother       Date:  2015-05-26       Impact factor: 5.191

6.  Selection of parasites with diminished drug susceptibility by amodiaquine-containing antimalarial regimens in Uganda.

Authors:  Fatima Nawaz; Samuel L Nsobya; Moses Kiggundu; Moses Joloba; Philip J Rosenthal
Journal:  J Infect Dis       Date:  2009-12-01       Impact factor: 5.226

7.  Dynamics of pfcrt alleles CVMNK and CVIET in chloroquine-treated Sudanese patients infected with Plasmodium falciparum.

Authors:  Nahla B Gadalla; Salah Eldin Elzaki; Ebtihal Mukhtar; David C Warhurst; Badria El-Sayed; Colin J Sutherland
Journal:  Malar J       Date:  2010-03-12       Impact factor: 2.979

8.  In vitro sensitivities of Plasmodium falciparum to different antimalarial drugs in Uganda.

Authors:  Samuel L Nsobya; Moses Kiggundu; Sarah Nanyunja; Moses Joloba; Bryan Greenhouse; Philip J Rosenthal
Journal:  Antimicrob Agents Chemother       Date:  2010-01-11       Impact factor: 5.191

9.  Optimization of a ligase detection reaction-fluorescent microsphere assay for characterization of resistance-mediating polymorphisms in African samples of Plasmodium falciparum.

Authors:  Norbert P LeClair; Melissa D Conrad; Frederick N Baliraine; Christian Nsanzabana; Samuel L Nsobya; Philip J Rosenthal
Journal:  J Clin Microbiol       Date:  2013-05-29       Impact factor: 5.948

10.  Competition between Plasmodium falciparum strains in clinical infections during in vitro culture adaptation.

Authors:  Kexuan Chen; Ling Sun; Yingxue Lin; Qi Fan; Zhenjun Zhao; Mingming Hao; Guohua Feng; Yanrui Wu; Liwang Cui; Zhaoqing Yang
Journal:  Infect Genet Evol       Date:  2014-03-22       Impact factor: 3.342
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