| Literature DB >> 1879704 |
Abstract
A shuttle expression vector containing the thiostrepton-inducible Streptomyces lividans promoter, ptipA, and the origin of transfer from plasmid RP4 was constructed. Cassettes containing a promoterless xylE gene upstream from a hyg gene were used to demonstrate thiostrepton-inducible expression from ptipA in both S. lividans and Streptomyces ambofaciens, ptipA was estimated to be induced 60-fold or more in Streptomyces ambofaciens.Entities:
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Year: 1991 PMID: 1879704 DOI: 10.1016/0378-1119(91)90398-u
Source DB: PubMed Journal: Gene ISSN: 0378-1119 Impact factor: 3.688