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Literature DB >> 1653214

Transposition of Tn5096 from a temperature-sensitive transducible plasmid in Streptomyces spp.

Abstract

Transposon Tn5096 was inserted into a derivative of the temperature-sensitive plasmid pMT660 containing the bacteriophage FP43 pac site. The resulting plasmid, pRHB126, was transduced by FP43 into several Streptomyces species. Tn5096 transposed from pRHB126 into different sites in the genomes of Streptomyces ambofaciens, Streptomyces cinnamonensis, Streptomyces coelicolor A3(2), Streptomyces fradiae, Streptomyces griseofuscus, and Streptomyces thermotolerans.

Entities: Species

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Year: 1991 PMID： 1653214 PMCID： PMC208276 DOI： 10.1128/jb.173.17.5578-5581.1991

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

15 in total

1. Transduction of plasmid DNA in Streptomyces spp. and related genera by bacteriophage FP43.

Authors: M A McHenney; R H Baltz
Journal: J Bacteriol Date: 1988-05 Impact factor: 3.490

2. Transduction of plasmid DNA in macrolide producing streptomycetes.

Authors: M A McHenney; R H Baltz
Journal: J Antibiot (Tokyo) Date: 1989-11 Impact factor: 2.649

3. Restriction of bacteriophage plaque formation in Streptomyces spp.

Authors: K L Cox; R H Baltz
Journal: J Bacteriol Date: 1984-08 Impact factor: 3.490

4. Method for selection of transposable DNA and characterization of a new insertion sequence, IS493, from Streptomyces lividans.

Authors: P J Solenberg; S G Burgett
Journal: J Bacteriol Date: 1989-09 Impact factor: 3.490

5. Transduction and transformation of plasmid DNA in Streptomyces fradiae strains that express different levels of restriction.

Authors: P Matsushima; M A McHenney; R H Baltz
Journal: J Bacteriol Date: 1989-06 Impact factor: 3.490

6. Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration.

Authors: R H Baltz
Journal: J Gen Microbiol Date: 1978-07

7. Efficient plasmid transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts.

Authors: P Matsushima; R H Baltz
Journal: J Bacteriol Date: 1985-07 Impact factor: 3.490

8. Production of a hybrid macrolide antibiotic in Streptomyces ambofaciens and Streptomyces lividans by introduction of a cloned carbomycin biosynthetic gene from Streptomyces thermotolerans.

Authors: J K Epp; M L Huber; J R Turner; T Goodson; B E Schoner
Journal: Gene Date: 1989-12-28 Impact factor: 3.688

Transposition of Tn5096 from a temperature-sensitive transducible plasmid in Streptomyces spp.

1. Transduction of plasmid DNA in Streptomyces spp. and related genera by bacteriophage FP43.

2. Transduction of plasmid DNA in macrolide producing streptomycetes.

3. Restriction of bacteriophage plaque formation in Streptomyces spp.

4. Method for selection of transposable DNA and characterization of a new insertion sequence, IS493, from Streptomyces lividans.

5. Transduction and transformation of plasmid DNA in Streptomyces fradiae strains that express different levels of restriction.

6. Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration.

7. Efficient plasmid transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts.

8. Production of a hybrid macrolide antibiotic in Streptomyces ambofaciens and Streptomyces lividans by introduction of a cloned carbomycin biosynthetic gene from Streptomyces thermotolerans.

9. Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.

10. Temperature-sensitive mutants of the Streptomyces plasmid pIJ702.

Review 1. Streptomyces and Saccharopolyspora hosts for heterologous expression of secondary metabolite gene clusters.

2. Establishment of Tn5096-based transposon mutagenesis in Gordonia polyisoprenivorans.

3. Tn5099, a xylE promoter probe transposon for Streptomyces spp.