Functional and structural units in the chromomere.

Electron microscopic observations demonstrate the existence of several DNA packing levels in the chromomere. A linear DNA molecule forms a big (chromomere) loop anchored to the chromosomal scaffold. The loop forms a set of smaller loops in the rosette pattern. Packing of the DNA by the histone octamer particles results in nucleosomes and nucleomeres. To establish the possible correspondence between the structural units of a chromomere and the genetical units (genes, exons, introns) in it, we compared the lengths of the units. Statistical analysis of the 315 sequenced genes indicate that the average gene size corresponds to the average length of a rosette loop. It means that a chromomere contains one or more genes. Assuming that exon-intron boundaries cannot bind nucleosomes we constructed DNA-packing models of the 88 genes. They demonstrate that the first (in 77.8 per cent of the genes) and the last (in 52.7 per cent) exons of the genes are too short to bind nucleosomes. Many genes contain long (nucleosome binding) pieces of DNA. Long packed pieces are introns in vertebrates; they are exons in invertebrates and plants. The average size gene contains two nucleomeres.