| Literature DB >> 18794860 |
Julie S Biteen1, Michael A Thompson, Nicole K Tselentis, Grant R Bowman, Lucy Shapiro, W E Moerner.
Abstract
The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.Entities:
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Year: 2008 PMID： 18794860 PMCID： PMC2655310 DOI： 10.1038/nmeth.1258
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547