OBJECTIVE: The gene coding for C-reactive protein (CRP) is located on chromosome 1q23.2, which falls within a linkage region thought to harbor a systemic lupus erythematosus (SLE) susceptibility gene. Recently, 2 single-nucleotide polymorphisms (SNP) in the CRP gene (+838, +2043) have been shown to be associated with CRP concentrations and/or SLE risk in a British family-based cohort. Our study was done to confirm the reported association in an independent population-based case-control cohort, and also to investigate the influence of 3 additional CRP tagSNP (-861, -390, +90) on SLE risk and serum CRP concentrations. METHODS: DNA from 337 Caucasian women who met the American College of Rheumatology criteria for definite (n = 324) or probable (n = 13) SLE and 448 Caucasian healthy female controls was genotyped for 5 CRP tagSNP (-861, -390, +90, +838, +2043). Genotyping was performed using restriction fragment length polymorphism-polymerase chain reaction, pyrosequencing, or TaqMan assays. Serum CRP levels were measured using ELISA. Association studies were performed using the chi-squared distribution, Z-test, Fisher's exact test, and analysis of variance. Haplotype analysis was performed using EH software and the haplo.stats package in R 2.1.2. RESULTS: While none of the SNP were found to be associated with SLE risk individually, there was an association with the 5 SNP haplotypes (p < 0.001). Three SNP (-861, -390, +90) were found to significantly influence serum CRP level in SLE cases, both independently and as haplotypes. CONCLUSION: Our data suggest that unique haplotype combinations in the CRP gene may modify the risk of developing SLE and influence circulating CRP levels.
OBJECTIVE: The gene coding for C-reactive protein (CRP) is located on chromosome 1q23.2, which falls within a linkage region thought to harbor a systemic lupus erythematosus (SLE) susceptibility gene. Recently, 2 single-nucleotide polymorphisms (SNP) in the CRP gene (+838, +2043) have been shown to be associated with CRP concentrations and/or SLE risk in a British family-based cohort. Our study was done to confirm the reported association in an independent population-based case-control cohort, and also to investigate the influence of 3 additional CRP tagSNP (-861, -390, +90) on SLE risk and serum CRP concentrations. METHODS: DNA from 337 Caucasian women who met the American College of Rheumatology criteria for definite (n = 324) or probable (n = 13) SLE and 448 Caucasian healthy female controls was genotyped for 5 CRP tagSNP (-861, -390, +90, +838, +2043). Genotyping was performed using restriction fragment length polymorphism-polymerase chain reaction, pyrosequencing, or TaqMan assays. Serum CRP levels were measured using ELISA. Association studies were performed using the chi-squared distribution, Z-test, Fisher's exact test, and analysis of variance. Haplotype analysis was performed using EH software and the haplo.stats package in R 2.1.2. RESULTS: While none of the SNP were found to be associated with SLE risk individually, there was an association with the 5 SNP haplotypes (p < 0.001). Three SNP (-861, -390, +90) were found to significantly influence serum CRP level in SLE cases, both independently and as haplotypes. CONCLUSION: Our data suggest that unique haplotype combinations in the CRP gene may modify the risk of developing SLE and influence circulating CRP levels.
Authors: Donato Alarcón-Segovia; Marta E Alarcón-Riquelme; Mario H Cardiel; Francisco Caeiro; Loreto Massardo; Antonio R Villa; Bernardo A Pons-Estel Journal: Arthritis Rheum Date: 2005-04
Authors: E V Barnes; S Narain; A Naranjo; J Shuster; M S Segal; E S Sobel; A E Armstrong; B E Santiago; W H Reeves; H B Richards Journal: Lupus Date: 2005 Impact factor: 2.911
Authors: A J Szalai; J Wu; E M Lange; M A McCrory; C D Langefeld; A Williams; S O Zakharkin; V George; D B Allison; G S Cooper; F Xie; Z Fan; J C Edberg; R P Kimberly Journal: J Mol Med (Berl) Date: 2005-03-19 Impact factor: 4.599
Authors: D T Miller; R Y L Zee; J Suk Danik; P Kozlowski; D I Chasman; R Lazarus; N R Cook; P M Ridker; D J Kwiatkowski Journal: Ann Hum Genet Date: 2005-11 Impact factor: 1.670
Authors: Christopher S Carlson; Shelley Force Aldred; Philip K Lee; Russell P Tracy; Stephen M Schwartz; Mark Rieder; Kiang Liu; O Dale Williams; Carlos Iribarren; E Cora Lewis; Myriam Fornage; Eric Boerwinkle; Myron Gross; Cashell Jaquish; Deborah A Nickerson; Richard M Myers; David S Siscovick; Alexander P Reiner Journal: Am J Hum Genet Date: 2005-05-16 Impact factor: 11.025
Authors: K L Moser; B R Neas; J E Salmon; H Yu; C Gray-McGuire; N Asundi; G R Bruner; J Fox; J Kelly; S Henshall; D Bacino; M Dietz; R Hogue; G Koelsch; L Nightingale; T Shaver; N I Abdou; D A Albert; C Carson; M Petri; E L Treadwell; J A James; J B Harley Journal: Proc Natl Acad Sci U S A Date: 1998-12-08 Impact factor: 11.205
Authors: Chi Chiu Mok; Daniel J Birmingham; Ling Yin Ho; Lee A Hebert; Brad H Rovin Journal: Arthritis Care Res (Hoboken) Date: 2013-03 Impact factor: 4.794
Authors: Christian Gytz Ammitzbøll; Rudi Steffensen; Martin Bøgsted; Kim Hørslev-Petersen; Merete L Hetland; Peter Junker; Julia S Johansen; Jan Pødenphant; Mikkel Østergaard; Torkell Ellingsen; Kristian Stengaard-Pedersen Journal: Arthritis Res Ther Date: 2014-10-31 Impact factor: 5.156