OBJECTIVE: The present investigation aimed to study the susceptibility of lymphocytes collected from brain tumour patients to radiation-induced DNA damage under in vitro conditions. METHODS: The peripheral lymphocytes collected from brain tumour patients were exposed to 2-Gy gamma radiation. Susceptibility of lymphocytes to radiation-induced DNA damage and their repair ability was assessed by alkaline comet assay. RESULTS: Lymphocytes of patients with benign and malignant tumour had a significantly higher (p < 0.001) baseline DNA damage compared to lymphocytes from normal subjects. A significant increase (p < 0.001) in DNA damage was observed immediately after irradiation of lymphocytes from healthy subjects and brain tumour patients. However, at 1 h after irradiation the level of DNA damage dropped significantly (p < 0.001) compared to that of immediately after irradiation of respective groups. DISCUSSION: In conclusion, the lymphocytes of brain tumour patients possess a higher level of basal DNA damage and exhibit a higher susceptibility to a clastogenic agent like radiation.
OBJECTIVE: The present investigation aimed to study the susceptibility of lymphocytes collected from brain tumourpatients to radiation-induced DNA damage under in vitro conditions. METHODS: The peripheral lymphocytes collected from brain tumourpatients were exposed to 2-Gy gamma radiation. Susceptibility of lymphocytes to radiation-induced DNA damage and their repair ability was assessed by alkaline comet assay. RESULTS: Lymphocytes of patients with benign and malignant tumour had a significantly higher (p < 0.001) baseline DNA damage compared to lymphocytes from normal subjects. A significant increase (p < 0.001) in DNA damage was observed immediately after irradiation of lymphocytes from healthy subjects and brain tumourpatients. However, at 1 h after irradiation the level of DNA damage dropped significantly (p < 0.001) compared to that of immediately after irradiation of respective groups. DISCUSSION: In conclusion, the lymphocytes of brain tumourpatients possess a higher level of basal DNA damage and exhibit a higher susceptibility to a clastogenic agent like radiation.
Authors: Jenny Chang-Claude; Odilia Popanda; Xiang-Lin Tan; Silke Kropp; Irmgard Helmbold; Dietrich von Fournier; Wulf Haase; Marie Luise Sautter-Bihl; Frederik Wenz; Peter Schmezer; Christine B Ambrosone Journal: Clin Cancer Res Date: 2005-07-01 Impact factor: 12.531
Authors: R El-Zein; M L Bondy; L E Wang; M de Andrade; A J Sigurdson; J M Bruner; A P Kyritsis; V A Levin; Q Wei Journal: Carcinogenesis Date: 1999-05 Impact factor: 4.944
Authors: M L Bondy; A P Kyritsis; J Gu; M de Andrade; J Cunningham; V A Levin; J M Bruner; Q Wei Journal: Cancer Res Date: 1996-04-01 Impact factor: 12.701
Authors: Serap Erdal; Bridget J McCarthy; Natalia Gurule; Marianne Berwick; Emily Gonzales; Johanna Byrd; Kristina Flores; JoAnna Shimek; Dora Il'yasova; Francis Ali-Osman; Darell D Bigner; Faith G Davis; Alexis N Leyba; Kirsten A M White Journal: Toxicol Rep Date: 2018-01-09