Literature DB >> 18791011

Simple and safe method for simultaneous isolation of microbial RNA and DNA from problematic populations.

Simon McIlroy1, Kate Porter, Robert J Seviour, Daniel Tillett.   

Abstract

We describe a novel, rapid, and safe method for extracting RNA and DNA from refractory microbes, which avoids the use of phenol or chloroform. It has been used successfully to isolate high-quality nucleic acids from pure cultures and environmental populations known to resist widely used extraction protocols.

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Year:  2008        PMID： 18791011      PMCID： PMC2576689          DOI： 10.1128/AEM.01047-08

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  13 in total

1.  Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition.

Authors:  R I Griffiths; A S Whiteley; A G O'Donnell; M J Bailey
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

2.  Involvement of Rhodocyclus-related organisms in phosphorus removal in full-scale wastewater treatment plants.

Authors:  Julie L Zilles; Jordan Peccia; Myeong-Woon Kim; Chun-Hsiung Hung; Daniel R Noguera
Journal:  Appl Environ Microbiol       Date:  2002-06       Impact factor: 4.792

3.  The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set.

Authors:  H Daims; A Brühl; R Amann; K H Schleifer; M Wagner
Journal:  Syst Appl Microbiol       Date:  1999-09       Impact factor: 4.022

4.  The microbial community composition of a nitrifying-denitrifying activated sludge from an industrial sewage treatment plant analyzed by the full-cycle rRNA approach.

Authors:  Stefan Juretschko; Alexander Loy; Angelika Lehner; Michael Wagner
Journal:  Syst Appl Microbiol       Date:  2002-04       Impact factor: 4.022

5.  Ecology of the microbial community removing phosphate from wastewater under continuously aerobic conditions in a sequencing batch reactor.

Authors:  Johwan Ahn; Sarah Schroeder; Michael Beer; Simon McIlroy; Ronald C Bayly; John W May; George Vasiliadis; Robert J Seviour
Journal:  Appl Environ Microbiol       Date:  2007-02-09       Impact factor: 4.792

Review 6.  Phylogenetic identification and in situ detection of individual microbial cells without cultivation.

Authors:  R I Amann; W Ludwig; K H Schleifer
Journal:  Microbiol Rev       Date:  1995-03

Review 7.  Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis.

Authors:  F von Wintzingerode; U B Göbel; E Stackebrandt
Journal:  FEMS Microbiol Rev       Date:  1997-11       Impact factor: 16.408

8.  Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation.

Authors:  G R Crocetti; P Hugenholtz; P L Bond; A Schuler; J Keller; D Jenkins; L L Blackall
Journal:  Appl Environ Microbiol       Date:  2000-03       Impact factor: 4.792

9.  Flow sorting of microorganisms for molecular analysis.

Authors:  G Wallner; B Fuchs; S Spring; W Beisker; R Amann
Journal:  Appl Environ Microbiol       Date:  1997-11       Impact factor: 4.792

10.  A rapid method for preparation of bacterial plasmids.

Authors:  J Summerton; T Atkins; R Bestwick
Journal:  Anal Biochem       Date:  1983-08       Impact factor: 3.365
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  5 in total

1.  Filamentous bacterium Eikelboom type 0092 in activated sludge plants in Australia is a member of the phylum Chloroflexi.

Authors:  Lachlan Speirs; Tadashi Nittami; Simon McIlroy; Sarah Schroeder; Robert J Seviour
Journal:  Appl Environ Microbiol       Date:  2009-02-13       Impact factor: 4.792

2.  Development of an Integrated Pipeline for Profiling Microbial Proteins from Mouse Fecal Samples by LC-MS/MS.

Authors:  Jing Wu; Jianhui Zhu; Haidi Yin; Xinhua Liu; Mingrui An; Nicholas A Pudlo; Eric C Martens; Grace Y Chen; David M Lubman
Journal:  J Proteome Res       Date:  2016-09-02       Impact factor: 4.466

3.  Diverse sulfate-reducing bacteria of the Desulfosarcina/Desulfococcus clade are the key alkane degraders at marine seeps.

Authors:  Sara Kleindienst; Florian-Alexander Herbst; Marion Stagars; Frederick von Netzer; Martin von Bergen; Jana Seifert; Jörg Peplies; Rudolf Amann; Florin Musat; Tillmann Lueders; Katrin Knittel
Journal:  ISME J       Date:  2014-04-10       Impact factor: 10.302

4.  Effect of sludge age on methanogenic and glycogen accumulating organisms in an aerobic granular sludge process fed with methanol and acetate.

Authors:  M Pronk; B Abbas; R Kleerebezem; M C M van Loosdrecht
Journal:  Microb Biotechnol       Date:  2015-06-08       Impact factor: 5.813

5.  Distinct Bacterial Communities in Surficial Seafloor Sediments Following the 2010 Deepwater Horizon Blowout.

Authors:  Tingting Yang; Kelly Speare; Luke McKay; Barbara J MacGregor; Samantha B Joye; Andreas Teske
Journal:  Front Microbiol       Date:  2016-09-13       Impact factor: 5.640
  5 in total

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