Literature DB >> 18788912

Analysis of nevirapine resistance mutations in cloned HIV type 1 variants from HIV-infected Ugandan infants using a single-step amplification-sequencing method (AmpliSeq).

William Ian Towler1, Jessica D Church, James R Eshleman, Mary Glenn Fowler, Laura A Guay, J Brooks Jackson, Susan H Eshleman.   

Abstract

We analyzed the genetic linkage of nevirapine (NVP) resistance mutations and the genetic complexity of HIV-1 variants in Ugandan infants who were HIV infected despite single dose (SD) prophylaxis. Plasma samples were obtained from six HIV-infected infants who had two or more NVP resistance mutations detected by population sequencing (ViroSeq). ViroSeq PCR products were cloned and transformed, and a single-step amplification-sequencing reaction (AmpliSeq) was used to analyze NVP resistance mutations in cloned HIV-1 variants directly from bacterial colonies. Fifty clones were analyzed for each infant sample. This analysis revealed numerous NVP resistance mutations not detected by population sequencing, genetically linked NVP resistance mutations, and a high degree of genetic complexity at codons that influence NVP susceptibility.

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Year:  2008        PMID： 18788912      PMCID： PMC2562759          DOI： 10.1089/aid.2008.0109

Source DB:  PubMed          Journal:  AIDS Res Hum Retroviruses        ISSN： 0889-2229            Impact factor:   2.205


  13 in total

1.  Analysis of HIV-1 variants by cloning DNA generated with the ViroSeq HIV-1 Genotyping System.

Authors:  Susan H Eshleman; Dana Jones; Tamara Flys; Olga Petrauskene; J Brooks Jackson
Journal:  Biotechniques       Date:  2003-09       Impact factor: 1.993

2.  Detection of minor drug-resistant populations by parallel allele-specific sequencing.

Authors:  Fangping Cai; Haifeng Chen; Charles B Hicks; John A Bartlett; Jun Zhu; Feng Gao
Journal:  Nat Methods       Date:  2007-01-07       Impact factor: 28.547

3.  Persistence of nevirapine-resistant HIV-1 in women after single-dose nevirapine therapy for prevention of maternal-to-fetal HIV-1 transmission.

Authors:  S Palmer; V Boltz; N Martinson; F Maldarelli; G Gray; J McIntyre; J Mellors; L Morris; J Coffin
Journal:  Proc Natl Acad Sci U S A       Date:  2006-04-25       Impact factor: 11.205

4.  Multiple, linked human immunodeficiency virus type 1 drug resistance mutations in treatment-experienced patients are missed by standard genotype analysis.

Authors:  Sarah Palmer; Mary Kearney; Frank Maldarelli; Elias K Halvas; Christian J Bixby; Holly Bazmi; Diane Rock; Judith Falloon; Richard T Davey; Robin L Dewar; Julia A Metcalf; Scott Hammer; John W Mellors; John M Coffin
Journal:  J Clin Microbiol       Date:  2005-01       Impact factor: 5.948

5.  Persistence of K103N-containing HIV-1 variants after single-dose nevirapine for prevention of HIV-1 mother-to-child transmission.

Authors:  Tamara S Flys; Deborah Donnell; Anthony Mwatha; Clemensia Nakabiito; Philippa Musoke; Francis Mmiro; J Brooks Jackson; Laura A Guay; Susan H Eshleman
Journal:  J Infect Dis       Date:  2007-01-18       Impact factor: 5.226

6.  Intrapartum and neonatal single-dose nevirapine compared with zidovudine for prevention of mother-to-child transmission of HIV-1 in Kampala, Uganda: HIVNET 012 randomised trial.

Authors:  L A Guay; P Musoke; T Fleming; D Bagenda; M Allen; C Nakabiito; J Sherman; P Bakaki; C Ducar; M Deseyve; L Emel; M Mirochnick; M G Fowler; L Mofenson; P Miotti; K Dransfield; D Bray; F Mmiro; J B Jackson
Journal:  Lancet       Date:  1999-09-04       Impact factor: 79.321

7.  Sequencing of genomic DNA by combined amplification and cycle sequencing reaction.

Authors:  Kathleen M Murphy; Karin D Berg; James R Eshleman
Journal:  Clin Chem       Date:  2004-10-28       Impact factor: 8.327

8.  Effectiveness of repeat single-dose nevirapine for prevention of mother-to-child transmission of HIV-1 in repeat pregnancies in Uganda.

Authors:  Michelle McConnell; Paul Bakaki; Chineta Eure; Michael Mubiru; Danstan Bagenda; Robert Downing; Flavia Matovu; Michael C Thigpen; Alan E Greenberg; Mary Glenn Fowler
Journal:  J Acquir Immune Defic Syndr       Date:  2007-11-01       Impact factor: 3.731

9.  Intrapartum and neonatal single-dose nevirapine compared with zidovudine for prevention of mother-to-child transmission of HIV-1 in Kampala, Uganda: 18-month follow-up of the HIVNET 012 randomised trial.

Authors:  J Brooks Jackson; Philippa Musoke; Thomas Fleming; Laura A Guay; Danstan Bagenda; Melissa Allen; Clemensia Nakabiito; Joseph Sherman; Paul Bakaki; Maxensia Owor; Constance Ducar; Martina Deseyve; Anthony Mwatha; Lynda Emel; Corey Duefield; Mark Mirochnick; Mary Glenn Fowler; Lynne Mofenson; Paolo Miotti; Maria Gigliotti; Dorothy Bray; Francis Mmiro
Journal:  Lancet       Date:  2003-09-13       Impact factor: 79.321

10.  Comparison of oligonucleotide ligation assay and consensus sequencing for detection of drug-resistant mutants of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and plasma.

Authors:  Giovanina M Ellis; Madhumita Mahalanabis; Ingrid A Beck; Gregory Pepper; Amy Wright; Shannon Hamilton; Sarah Holte; Willscott E Naugler; Diane M Pawluk; Chung-Chen Li; Lisa M Frenkel
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948
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  2 in total

1.  Analysis of HIV diversity using a high-resolution melting assay.

Authors:  William I Towler; Maria M James; Stuart C Ray; Lei Wang; Deborah Donnell; Anthony Mwatha; Laura Guay; Clemensia Nakabiito; Philippa Musoke; J Brooks Jackson; Susan H Eshleman
Journal:  AIDS Res Hum Retroviruses       Date:  2010-08       Impact factor: 2.205

2.  Development of elvitegravir resistance and linkage of integrase inhibitor mutations with protease and reverse transcriptase resistance mutations.

Authors:  Mark A Winters; Robert M Lloyd; Robert W Shafer; Michael J Kozal; Michael D Miller; Mark Holodniy
Journal:  PLoS One       Date:  2012-07-18       Impact factor: 3.240
  2 in total

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