Literature DB >> 18770732

DNA content measurement for DNA ploidy and cell cycle analysis.

Z Darzynkiewicz1, G Juan.   

Abstract

This unit presents the most common methods of DNA content analysis. The techniques differ primarily in the mode of cell permeabilization (detergents versus prefixation with alcohol) and composition of the stain solution. Protocols are given for analysis of fixed cells, tissue samples, live cells (for subsequent sorting and culture), archival paraffin-embedded tissue, and apoptotic cells.

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Year:  2001        PMID： 18770732     DOI： 10.1002/0471142956.cy0705s00

Source DB:  PubMed          Journal:  Curr Protoc Cytom        ISSN： 1934-9297


  31 in total

1.  A rapid and optimization-free procedure allows the in vivo detection of subtle cell cycle and ploidy alterations in tissues by flow cytometry.

Authors:  Christina Heinlein; Wolfgang Deppert; Antony W Braithwaite; Daniel Speidel
Journal:  Cell Cycle       Date:  2010-09-01       Impact factor: 4.534

2.  Geminin deletion from hematopoietic cells causes anemia and thrombocytosis in mice.

Authors:  Kathryn M Shinnick; Elizabeth A Eklund; Thomas J McGarry
Journal:  J Clin Invest       Date:  2010-12       Impact factor: 14.808

3.  Chromatin organization measured by AluI restriction enzyme changes with malignancy and is regulated by the extracellular matrix and the cytoskeleton.

Authors:  Andrew J Maniotis; Klara Valyi-Nagy; John Karavitis; Jonas Moses; Viveka Boddipali; Ying Wang; Rafael Nuñez; Suman Setty; Zarema Arbieva; Mina J Bissell; Robert Folberg
Journal:  Am J Pathol       Date:  2005-04       Impact factor: 4.307

4.  Exocrine pancreatic disorders in transsgenic mice expressing human keratin 8.

Authors:  M L Casanova; A Bravo; A Ramírez; G Morreale de Escobar; F Were; G Merlino; M Vidal; J L Jorcano
Journal:  J Clin Invest       Date:  1999-06       Impact factor: 14.808

5.  Role of DNA Methylation on the Expression of the Anthracycline Metabolizing Enzyme AKR7A2 in Human Heart.

Authors:  Carrie C Hoefer; Adolfo Quiñones-Lombraña; Rachael Hageman Blair; Javier G Blanco
Journal:  Cardiovasc Toxicol       Date:  2016-04       Impact factor: 3.231

6.  Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts.

Authors:  Susumu Ohshima; Atsushi Seyama
Journal:  J Vis Exp       Date:  2017-01-08       Impact factor: 1.355

7.  Enucleation of feeder cells and egg cells with psoralens.

Authors:  Thomas J McGarry; Michael Bonaguidi; Ljuba Lyass; John A Kessler; Jason M Bodily; Lynn Doglio
Journal:  Dev Dyn       Date:  2009-10       Impact factor: 3.780

8.  A Live-Cell Screen for Altered Erk Dynamics Reveals Principles of Proliferative Control.

Authors:  Alexander G Goglia; Maxwell Z Wilson; Siddhartha G Jena; Jillian Silbert; Lena P Basta; Danelle Devenport; Jared E Toettcher
Journal:  Cell Syst       Date:  2020-03-18       Impact factor: 10.304

9.  Long intergenic non-coding RNA HOTAIRM1 regulates cell cycle progression during myeloid maturation in NB4 human promyelocytic leukemia cells.

Authors:  Xueqing Zhang; Sherman M Weissman; Peter E Newburger
Journal:  RNA Biol       Date:  2014-04-24       Impact factor: 4.652

10.  Inducing triploids and tetraploids with high temperatures in Populus sect. Tacamahaca.

Authors:  Liqin Guo; Wenting Xu; Yan Zhang; Jinfeng Zhang; Zunzheng Wei
Journal:  Plant Cell Rep       Date:  2016-11-17       Impact factor: 4.570
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