| Literature DB >> 18751903 |
Lutfan Lazuardi1, Dietmar Herndler-Brandstetter, Stefan Brunner, Gerhard T Laschober, Günter Lepperdinger, Beatrix Grubeck-Loebenstein.
Abstract
We isolated highly purified CD8+CD28+ and CD8+CD28- T cell populations from healthy young and elderly persons for gene expression profiling using Affymetrix oligonucleotide microarrays. We demonstrate that the gene expression profile of CD8+CD28- T cells is very similar in young and elderly persons. In contrast, CD8+CD28+ in elderly differ from CD8+CD28+ in young persons. Hierarchical clustering revealed that CD8+CD28+ in elderly are located between CD8+CD28+ in young and CD8+CD28- (young and old) T cells regarding their differentiation state. Our study demonstrates a dichotomy of gene expression levels between CD8+CD28+ T cells in young and elderly persons but a similarity between CD8+CD28- T cells in young and elderly persons. As CD8+CD28+ T cells from elderly and young persons are distinct due to a different composition of the population, these results suggest that the gene expression profile does not depend on chronological age but depends on the differentiation state of the individual cell types.Entities:
Mesh：
Substances：
Year: 2008 PMID： 18751903 DOI： 10.1007/s10522-008-9167-1
Source DB: PubMed Journal: Biogerontology ISSN： 1389-5729 Impact factor: 4.277