| Literature DB >> 187176 |
Abstract
The interaction of pigeon liver NAD kinase with Cibacron Blue F3GA was investigated. By using steady-state rate measurements, spectrophotometric titration and chromatography of the enzyme on immobilized dye, it was shown that binding occurs at two nucleotide sites with different affinities, and also at a site distinct from the substrate-binding region.Entities:
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Year: 1976 PMID: 187176 PMCID: PMC1164131 DOI: 10.1042/bj1590441
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857