| Literature DB >> 18711140 |
Paul P Szotek1, Henry L Chang, Kristen Brennand, Akihiro Fujino, Rafael Pieretti-Vanmarcke, Cristina Lo Celso, David Dombkowski, Frederic Preffer, Kenneth S Cohen, Jose Teixeira, Patricia K Donahoe.
Abstract
Ovulation induces cyclic rupture and regenerative repair of the ovarian coelomic epithelium. This process of repeated disruption and repair accompanied by complex remodeling typifies a somatic stem/progenitor cell-mediated process. Using BrdU incorporation and doxycycline inducible histone2B-green fluorescent protein pulse-chase techniques, we identify a label-retaining cell population in the coelomic epithelium of the adult mouse ovary as candidate somatic stem/progenitor cells. The identified population exhibits quiescence with asymmetric label retention, functional response to estrous cycling in vivo by proliferation, enhanced growth characteristics by in vitro colony formation, and cytoprotective mechanisms by enrichment for the side population. Together, these characteristics identify the label-retaining cell population as a candidate for the putative somatic stem/progenitor cells of the coelomic epithelium of the mouse ovary.Entities:
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Year: 2008 PMID： 18711140 PMCID： PMC2527935 DOI： 10.1073/pnas.0805012105
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205