| Literature DB >> 18710284 |
Gerhard Lang1, Nor Ainy Mayhudin, Maya I Mitova, Lin Sun, Sonia van der Sar, John W Blunt, Anthony L J Cole, Gill Ellis, Hartmut Laatsch, Murray H G Munro.
Abstract
The use of an HPLC bioactivity profiling/microtiter plate technique in conjunction with capillary probe NMR instrumentation and access to appropriate databases effectively short-circuits conventional dereplication procedures, necessarily based on multimilligram extracts, to a single, more rapid submilligram operation. This approach to dereplication is illustrated using fungal or bacterial extracts that contain known compounds. In each case the dereplication steps were carried out on microgram quantities of extract and demonstrate the discriminating power of (1)H NMR spectroscopy as a definitive dereplication tool.Mesh：
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Year: 2008 PMID： 18710284 DOI： 10.1021/np8002222
Source DB: PubMed Journal: J Nat Prod ISSN： 0163-3864 Impact factor: 4.050