Literature DB >> 18707900

Comparison of LC and LC/MS methods for quantifying N-glycosylation in recombinant IgGs.

Sandipan Sinha1, Gary Pipes, Elizabeth M Topp, Pavel V Bondarenko, Michael J Treuheit, Himanshu S Gadgil.   

Abstract

High-performance liquid chromatography (LC) and liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-MS) methods with various sample preparation schemes were compared for their ability to identify and quantify glycoforms in two different production lots of a recombinant monoclonal IgG1 antibody. IgG1s contain a conserved N-glycosylation site in the fragment crystallizable (Fc) subunit. Six methods were compared: (1) LC/ESI-MS analysis of intact IgG, (2) LC/ESI-MS analysis of the Fc fragment produced by limited proteolysis with Lys-C, (3) LC/ESI-MS analysis of the IgG heavy chain produced by reduction, (4) LC/ESI-MS analysis of Fc/2 fragment produced by limited proteolysis and reduction, (5) LC/MS analysis of the glycosylated tryptic fragment (293EEQYNSTYR301) using extracted ion chromatograms, and (6) normal phase HPLC analysis of N-glycans cleaved from the IgG using PNGase F. The results suggest that MS quantitation based on the analysis of Fc/2 (4) is accurate and gives results that are comparable to normal phase HPLC analysis of N-glycans (6).

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Year:  2008        PMID： 18707900     DOI： 10.1016/j.jasms.2008.07.004

Source DB:  PubMed          Journal:  J Am Soc Mass Spectrom        ISSN： 1044-0305            Impact factor:   3.109


  23 in total

1.  Validation of a peptide mapping method for a therapeutic monoclonal antibody: what could we possibly learn about a method we have run 100 times?

Authors:  J Bongers; J J Cummings; M B Ebert; M M Federici; L Gledhill; D Gulati; G M Hilliard; B H Jones; K R Lee; J Mozdzanowski; M Naimoli; S Burman
Journal:  J Pharm Biomed Anal       Date:  2000-01       Impact factor: 3.935

2.  Improving mass accuracy of high performance liquid chromatography/electrospray ionization time-of-flight mass spectrometry of intact antibodies.

Authors:  Himanshu S Gadgil; Gary D Pipes; Thomas M Dillon; Michael J Treuheit; Pavel V Bondarenko
Journal:  J Am Soc Mass Spectrom       Date:  2006-04-21       Impact factor: 3.109

3.  Kinetics of whole serum and prepurified IgG digestion by pepsin for F(ab')2 manufacture.

Authors:  Rihab Boushaba; Peter Kumpalume; Nigel K H Slater
Journal:  Biotechnol Prog       Date:  2003 Jul-Aug

Review 4.  Protein glycosylation analysis by liquid chromatography-mass spectrometry.

Authors:  Manfred Wuhrer; André M Deelder; Cornelis H Hokke
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2005-10-25       Impact factor: 3.205

5.  Identification of cysteinylation of a free cysteine in the Fab region of a recombinant monoclonal IgG1 antibody using Lys-C limited proteolysis coupled with LC/MS analysis.

Authors:  Himanshu S Gadgil; Pavel V Bondarenko; Gary D Pipes; Thomas M Dillon; Douglas Banks; Jeffrey Abel; Gerd R Kleemann; Michael J Treuheit
Journal:  Anal Biochem       Date:  2006-06-15       Impact factor: 3.365

6.  Metabolic control of recombinant monoclonal antibody N-glycosylation in GS-NS0 cells.

Authors:  A E Hills; A Patel; P Boyd; D C James
Journal:  Biotechnol Bioeng       Date:  2001-10-20       Impact factor: 4.530

7.  Reversed-phase liquid chromatography of immunoglobulin G molecules and their fragments with the diphenyl column.

Authors:  Da Ren; Gary D Pipes; David M Hambly; Pavel V Bondarenko; Michael J Treuheit; David N Brems; Himanshu S Gadgil
Journal:  J Chromatogr A       Date:  2007-10-10       Impact factor: 4.759

8.  Role of glycosylation on the secretion and biological activity of erythropoietin.

Authors:  E Delorme; T Lorenzini; J Giffin; F Martin; F Jacobsen; T Boone; S Elliott
Journal:  Biochemistry       Date:  1992-10-20       Impact factor: 3.162

9.  Determination and characterization of site-specific N-glycosylation using MALDI-Qq-TOF tandem mass spectrometry: case study with a plant protease.

Authors:  Natalia V Bykova; Christof Rampitsch; Oleg Krokhin; Kenneth G Standing; Werner Ens
Journal:  Anal Chem       Date:  2006-02-15       Impact factor: 6.986

10.  The LC/MS analysis of glycation of IgG molecules in sucrose containing formulations.

Authors:  Himanshu S Gadgil; Pavel V Bondarenko; Gary Pipes; Doug Rehder; Arnold McAuley; Natalie Perico; Tom Dillon; Margaret Ricci; Michael Treuheit
Journal:  J Pharm Sci       Date:  2007-10       Impact factor: 3.534
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  23 in total

1.  Rapid comparison of a candidate biosimilar to an innovator monoclonal antibody with advanced liquid chromatography and mass spectrometry technologies.

Authors:  Hongwei Xie; Asish Chakraborty; Joomi Ahn; Ying Qing Yu; Deepalakshmi P Dakshinamoorthy; Martin Gilar; Weibin Chen; St John Skilton; Jeffery R Mazzeo
Journal:  MAbs       Date:  2010-07-01       Impact factor: 5.857

2.  Investigation of Sequence Clipping and Structural Heterogeneity of an HIV Broadly Neutralizing Antibody by a Comprehensive LC-MS Analysis.

Authors:  Vera B Ivleva; Nicole A Schneck; Deepika Gollapudi; Frank Arnold; Jonathan W Cooper; Q Paula Lei
Journal:  J Am Soc Mass Spectrom       Date:  2018-05-07       Impact factor: 3.109

3.  Electrospray ionization quadrupole ion-mobility time-of-flight mass spectrometry as a tool to distinguish the lot-to-lot heterogeneity in N-glycosylation profile of the therapeutic monoclonal antibody trastuzumab.

Authors:  Carola W N Damen; Weibin Chen; Asish B Chakraborty; Mike van Oosterhout; Jeffrey R Mazzeo; John C Gebler; Jan H M Schellens; Hilde Rosing; Jos H Beijnen
Journal:  J Am Soc Mass Spectrom       Date:  2009-08-07       Impact factor: 3.109

Review 4.  Structure, heterogeneity and developability assessment of therapeutic antibodies.

Authors:  Yingda Xu; Dongdong Wang; Bruce Mason; Tony Rossomando; Ning Li; Dingjiang Liu; Jason K Cheung; Wei Xu; Smita Raghava; Amit Katiyar; Christine Nowak; Tao Xiang; Diane D Dong; Joanne Sun; Alain Beck; Hongcheng Liu
Journal:  MAbs       Date:  2018-12-17       Impact factor: 5.857

5.  Antibody characterization using novel ERLIC-MS/MS-based peptide mapping.

Authors:  Jing Zhen; John Kim; Ying Zhou; Ervinas Gaidamauskas; Shyamsundar Subramanian; Ping Feng
Journal:  MAbs       Date:  2018-09-11       Impact factor: 5.857

Review 6.  High-sensitivity analytical approaches for the structural characterization of glycoproteins.

Authors:  William R Alley; Benjamin F Mann; Milos V Novotny
Journal:  Chem Rev       Date:  2013-03-27       Impact factor: 60.622

7.  A Method for Comprehensive Glycosite-Mapping and Direct Quantitation of Serum Glycoproteins.

Authors:  Qiuting Hong; L Renee Ruhaak; Carol Stroble; Evan Parker; Jincui Huang; Emanual Maverakis; Carlito B Lebrilla
Journal:  J Proteome Res       Date:  2015-11-09       Impact factor: 4.466

8.  Comparison of analytical methods for profiling N- and O-linked glycans from cultured cell lines : HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study.

Authors:  Hiromi Ito; Hiroyuki Kaji; Akira Togayachi; Parastoo Azadi; Mayumi Ishihara; Rudolf Geyer; Christina Galuska; Hildegard Geyer; Kazuaki Kakehi; Mitsuhiro Kinoshita; Niclas G Karlsson; Chunsheng Jin; Koichi Kato; Hirokazu Yagi; Sachiko Kondo; Nana Kawasaki; Noritaka Hashii; Daniel Kolarich; Kathrin Stavenhagen; Nicolle H Packer; Morten Thaysen-Andersen; Miyako Nakano; Naoyuki Taniguchi; Ayako Kurimoto; Yoshinao Wada; Michiko Tajiri; Pengyuan Yang; Weiqian Cao; Hong Li; Pauline M Rudd; Hisashi Narimatsu
Journal:  Glycoconj J       Date:  2015-10-28       Impact factor: 2.916

Review 9.  Analytical and Functional Aspects of Antibody Sialylation.

Authors:  Johannes Stadlmann; Martin Pabst; Friedrich Altmann
Journal:  J Clin Immunol       Date:  2010-05       Impact factor: 8.317

10.  Physical stability comparisons of IgG1-Fc variants: effects of N-glycosylation site occupancy and Asp/Gln residues at site Asn 297.

Authors:  Mohammad A Alsenaidy; Solomon Z Okbazghi; Jae Hyun Kim; Sangeeta B Joshi; C Russell Middaugh; Thomas J Tolbert; David B Volkin
Journal:  J Pharm Sci       Date:  2014-04-16       Impact factor: 3.534
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