Literature DB >> 18707542

Plant-produced hepatitis B core protein chimera carrying anthrax protective antigen domain-4.

Katarzyna Bandurska1, Robert Brodzik, Sergei Spitsin, Thomas Kohl, Carla Portocarrero, Yuri Smirnov, Natalia Pogrebnyak, Agnieszka Sirko, Hilary Koprowski, Maxim Golovkin.   

Abstract

The hepatitis B core antigen (HBcAg) can generate a strong immune response and is recognized as an effective carrier for foreign epitopes. The domain-4 epitope of the anthrax protective antigen (PA-D4) plays an essential role in generating protective immunity against virulent Bacillus anthracis. Here we report the successful production of a recombinant protein comprised of the antigenic PA-D4 integrated into the c/e1 loop of HBcAg in transgenic low-alkaloid Nicotiana tabacum. Sera of mice injected with the plant-derived purified HB/PA-D4 protein exhibited significant anti-PA- and anti-HBcAg-specific IgG titers; however, formation of virus-like particles (VLP) was not observed. These data support the feasibility of producing complex protein chimeras in plants.

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Year:  2008        PMID： 18707542     DOI： 10.1089/hyb.2008.0008

Source DB:  PubMed          Journal:  Hybridoma (Larchmt)        ISSN： 1554-0014


  8 in total

Review 1.  Two decades of plant-based candidate vaccines: a review of the chimeric protein approaches.

Authors:  Ruth Elena Soria-Guerra; Leticia Moreno-Fierros; Sergio Rosales-Mendoza
Journal:  Plant Cell Rep       Date:  2011-04-20       Impact factor: 4.570

Review 2.  Expression of recombinant vaccines and antibodies in plants.

Authors:  Kisung Ko
Journal:  Monoclon Antib Immunodiagn Immunother       Date:  2014-06

Review 3.  The use of plants for the production of therapeutic human peptides.

Authors:  Chiara Lico; Luca Santi; Richard M Twyman; Mario Pezzotti; Linda Avesani
Journal:  Plant Cell Rep       Date:  2012-01-05       Impact factor: 4.964

Review 4.  Virus-like particles as a highly efficient vaccine platform: diversity of targets and production systems and advances in clinical development.

Authors:  Natasha Kushnir; Stephen J Streatfield; Vidadi Yusibov
Journal:  Vaccine       Date:  2012-11-06       Impact factor: 3.641

Review 5.  Plant-Based Vaccines in Combat against Coronavirus Diseases.

Authors:  Benita Ortega-Berlanga; Tomasz Pniewski
Journal:  Vaccines (Basel)       Date:  2022-01-18

Review 6.  A Comprehensive Overview on the Production of Vaccines in Plant-Based Expression Systems and the Scope of Plant Biotechnology to Combat against SARS-CoV-2 Virus Pandemics.

Authors:  Manu Kumar; Nisha Kumari; Nishant Thakur; Shashi Kant Bhatia; Ganesh Dattatraya Saratale; Gajanan Ghodake; Bhupendra M Mistry; Hemasundar Alavilli; D S Kishor; Xueshi Du; Sang-Min Chung
Journal:  Plants (Basel)       Date:  2021-06-15

Review 7.  Synthetic biology for bioengineering virus-like particle vaccines.

Authors:  Hayley K Charlton Hume; João Vidigal; Manuel J T Carrondo; Anton P J Middelberg; António Roldão; Linda H L Lua
Journal:  Biotechnol Bioeng       Date:  2018-12-31       Impact factor: 4.530

Review 8.  Plant-produced candidate countermeasures against emerging and reemerging infections and bioterror agents.

Authors:  Stephen J Streatfield; Natasha Kushnir; Vidadi Yusibov
Journal:  Plant Biotechnol J       Date:  2015-10       Impact factor: 9.803
  8 in total

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