PURPOSE: To investigate the healing of critical-size cranial bone defects (9-mm-diameter) in guinea pigs treated with a bovine bone-derived resorbable membrane. MATERIALS AND METHODS: A sample of 42 guinea pigs was divided into test (n = 20), control (n = 20), and standard (n = 2) groups. A full-thickness trephine defect was made in the fronto-parietal bone of each animal. In the test group, the internal and external openings of the defect were each closed with a separate membrane, and the space between them was filled with blood clot and a central spacer. In the control group, the defect was filled only with the blood clot and spacer. At 1, 3, 6, and 9 months later, the calvarias (5 per period) for both the test and control groups were collected, fixed, radiographed, and histologically processed. The standard-group animals were sacrificed immediately after surgery and used to determine the initial size of defect radiographically. The areas of defects in the radiographs were measured with image-analysis software and were compared between groups and periods by multiple regression analysis with the Bonferroni correction. RESULTS: At 1 and 3 months, newly formed woven bone was histologically observed in both test and control groups. Radiographically, this new bone occupied an average of 32% of the defect area at 1 month and 60% at 3 months in the test group. In the control group, 21% of the defect was filled at 1 month and 39% at 3 months. However, the differences between treatments were not statistically significant (P > .05). At 6 and 9 months, a significant increase in newly formed lamellar bone was seen histologically in both groups. Radiographically, for the test group, the new bone occupied an average of 82% of the defect area at 6 months and 96% at 9 months. For the control group, new bone composed an average of 45% of the defect area at 6 months and 40% at 9 months. The differences between the test and control groups were statistically significant at 6 and 9 months (P < .05). Complete or almost complete filling of the defect was observed in several cases. CONCLUSION: It was concluded that the bovine bone-derived membrane is highly biocompatible and is able to promote good healing of critical-size defects in calvaria of guinea pig.
PURPOSE: To investigate the healing of critical-size cranial bone defects (9-mm-diameter) in guinea pigs treated with a bovine bone-derived resorbable membrane. MATERIALS AND METHODS: A sample of 42 guinea pigs was divided into test (n = 20), control (n = 20), and standard (n = 2) groups. A full-thickness trephine defect was made in the fronto-parietal bone of each animal. In the test group, the internal and external openings of the defect were each closed with a separate membrane, and the space between them was filled with blood clot and a central spacer. In the control group, the defect was filled only with the blood clot and spacer. At 1, 3, 6, and 9 months later, the calvarias (5 per period) for both the test and control groups were collected, fixed, radiographed, and histologically processed. The standard-group animals were sacrificed immediately after surgery and used to determine the initial size of defect radiographically. The areas of defects in the radiographs were measured with image-analysis software and were compared between groups and periods by multiple regression analysis with the Bonferroni correction. RESULTS: At 1 and 3 months, newly formed woven bone was histologically observed in both test and control groups. Radiographically, this new bone occupied an average of 32% of the defect area at 1 month and 60% at 3 months in the test group. In the control group, 21% of the defect was filled at 1 month and 39% at 3 months. However, the differences between treatments were not statistically significant (P > .05). At 6 and 9 months, a significant increase in newly formed lamellar bone was seen histologically in both groups. Radiographically, for the test group, the new bone occupied an average of 82% of the defect area at 6 months and 96% at 9 months. For the control group, new bone composed an average of 45% of the defect area at 6 months and 40% at 9 months. The differences between the test and control groups were statistically significant at 6 and 9 months (P < .05). Complete or almost complete filling of the defect was observed in several cases. CONCLUSION: It was concluded that the bovine bone-derived membrane is highly biocompatible and is able to promote good healing of critical-size defects in calvaria of guinea pig.
Authors: Thais Accorsi-Mendonça; Willian Fernando Zambuzzi; Clóvis Monteiro Bramante; Tânia Mari Cestari; Rumio Taga; Márcia Sader; Glória Dulce de Almeida Soares; José Mauro Granjeiro Journal: J Mater Sci Mater Med Date: 2011-03-20 Impact factor: 3.896
Authors: Carlos Alberto Soriano-Souza; Andre L Rossi; Elena Mavropoulos; Moema A Hausen; Marcelo N Tanaka; Mônica D Calasans-Maia; Jose M Granjeiro; Maria Helena M Rocha-Leão; Alexandre M Rossi Journal: J Mater Sci Mater Med Date: 2015-03-20 Impact factor: 3.896
Authors: Lobna Abdel Aziz Aly; Hala El-Menoufy; Amal Hassan; Alyaa Ragae; Hazem Mahmoud Atta; Nagwa Kamal Roshdy; Laila Ahmed Rashed; Dina Sabry Journal: Int J Stem Cells Date: 2011-06 Impact factor: 2.500
Authors: Carolina Ferrairo Danieletto-Zanna; Vinícius Ferreira Bizelli; Guilherme André Del Arco Ramires; Tamires Melo Francatti; Paulo Sérgio Perri de Carvalho; Ana Paula Farnezi Bassi Journal: Int J Biomater Date: 2020-02-18