Literature DB >> 18691600

Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome.

Jonathan A R Worrall1, Maria Górna, Nicholas T Crump, Lara G Phillips, Alex C Tuck, Amanda J Price, Vassiliy N Bavro, Ben F Luisi.   

Abstract

The Escherichia coli RNA degradosome is a multienzyme assembly that functions in transcript turnover and maturation of structured RNA precursors. We have developed a procedure to reconstitute the RNA degradosome from recombinant components using modular coexpression vectors. The reconstituted assembly can be purified on a scale that has enabled biochemical and biophysical analyses, and we compare the properties of recombinant and cell-extracted RNA degradosomes. We present evidence that auxiliary protein components can be recruited to the 'superprotomer' core of the assembly through a dynamic equilibrium involving RNA intermediaries. We discuss the implications for the regulation of RNA degradosome function in vivo.

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 18691600      PMCID： PMC7611026          DOI： 10.1016/j.jmb.2008.07.059

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  53 in total

1.  Cold-temperature induction of Escherichia coli polynucleotide phosphorylase occurs by reversal of its autoregulation.

Authors:  R K Beran; R W Simons
Journal:  Mol Microbiol       Date:  2001-01       Impact factor: 3.501

2.  Escherichia coli RNA degradosome.

Authors:  A J Carpousis; A Leroy; N Vanzo; V Khemici
Journal:  Methods Enzymol       Date:  2001       Impact factor: 1.600

Review 3.  The long unwinding road of RNA helicases.

Authors:  Franziska Bleichert; Susan J Baserga
Journal:  Mol Cell       Date:  2007-08-03       Impact factor: 17.970

Review 4.  RNA helicases--one fold for many functions.

Authors:  Eckhard Jankowsky; Margaret E Fairman
Journal:  Curr Opin Struct Biol       Date:  2007-06-15       Impact factor: 6.809

5.  Copurification of E. coli RNAase E and PNPase: evidence for a specific association between two enzymes important in RNA processing and degradation.

Authors:  A J Carpousis; G Van Houwe; C Ehretsmann; H M Krisch
Journal:  Cell       Date:  1994-03-11       Impact factor: 41.582

6.  RNA components of Escherichia coli degradosome: evidence for rRNA decay.

Authors:  D A Bessarab; V R Kaberdin; C L Wei; G G Liou; S Lin-Chao
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-17       Impact factor: 11.205

7.  RNaseE and the other constituents of the RNA degradosome are components of the bacterial cytoskeleton.

Authors:  Aziz Taghbalout; Lawrence Rothfield
Journal:  Proc Natl Acad Sci U S A       Date:  2007-01-22       Impact factor: 11.205

8.  Regions of RNase E important for 5'-end-dependent RNA cleavage and autoregulated synthesis.

Authors:  X Jiang; A Diwa; J G Belasco
Journal:  J Bacteriol       Date:  2000-05       Impact factor: 3.490

9.  The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus.

Authors:  K J McDowall; R G Hernandez; S Lin-Chao; S N Cohen
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

10.  CsrA inhibits translation initiation of Escherichia coli hfq by binding to a single site overlapping the Shine-Dalgarno sequence.

Authors:  Carol S Baker; Lél A Eöry; Helen Yakhnin; Jeffrey Mercante; Tony Romeo; Paul Babitzke
Journal:  J Bacteriol       Date:  2007-05-25       Impact factor: 3.490
View more
  41 in total

1.  Characterization of the RNA degradosome of Pseudoalteromonas haloplanktis: conservation of the RNase E-RhlB interaction in the gammaproteobacteria.

Authors:  Soraya Aït-Bara; Agamemnon J Carpousis
Journal:  J Bacteriol       Date:  2010-08-20       Impact factor: 3.490

2.  Requirement of the CsdA DEAD-box helicase for low temperature riboregulation of rpoS mRNA.

Authors:  Armin Resch; Branislav Većerek; Kristina Palavra; Udo Bläsi
Journal:  RNA Biol       Date:  2010-11-01       Impact factor: 4.652

3.  Coding sequence targeting by MicC RNA reveals bacterial mRNA silencing downstream of translational initiation.

Authors:  Verena Pfeiffer; Kai Papenfort; Sacha Lucchini; Jay C D Hinton; Jörg Vogel
Journal:  Nat Struct Mol Biol       Date:  2009-07-20       Impact factor: 15.369

4.  Role of the five RNA helicases in the adaptive response of Bacillus cereus ATCC 14579 cells to temperature, pH, and oxidative stresses.

Authors:  Franck Pandiani; Stéphanie Chamot; Julien Brillard; Frédéric Carlin; Christophe Nguyen-the; Véronique Broussolle
Journal:  Appl Environ Microbiol       Date:  2011-06-24       Impact factor: 4.792

5.  RNase E action at a distance: degradation of target mRNAs mediated by an Hfq-binding small RNA in bacteria.

Authors:  Teppei Morita; Hiroji Aiba
Journal:  Genes Dev       Date:  2011-02-15       Impact factor: 11.361

Review 6.  Trans-acting regulators of ribonuclease activity.

Authors:  Jaejin Lee; Minho Lee; Kangseok Lee
Journal:  J Microbiol       Date:  2021-02-10       Impact factor: 3.422

7.  Regulation of ribonuclease E activity by the L4 ribosomal protein of Escherichia coli.

Authors:  Dharam Singh; Ssu-Jean Chang; Pei-Hsun Lin; Olga V Averina; Vladimir R Kaberdin; Sue Lin-Chao
Journal:  Proc Natl Acad Sci U S A       Date:  2009-01-14       Impact factor: 11.205

8.  Defining a role for Hfq in Gram-positive bacteria: evidence for Hfq-dependent antisense regulation in Listeria monocytogenes.

Authors:  Jesper Sejrup Nielsen; Lisbeth Kristensen Lei; Tine Ebersbach; Anders Steno Olsen; Janne Kudsk Klitgaard; Poul Valentin-Hansen; Birgitte Haahr Kallipolitis
Journal:  Nucleic Acids Res       Date:  2009-11-26       Impact factor: 16.971

9.  Crystal structure of Escherichia coli polynucleotide phosphorylase core bound to RNase E, RNA and manganese: implications for catalytic mechanism and RNA degradosome assembly.

Authors:  Salima Nurmohamed; Bhamini Vaidialingam; Anastasia J Callaghan; Ben F Luisi
Journal:  J Mol Biol       Date:  2009-03-24       Impact factor: 5.469

10.  Translational activation of rpoS mRNA by the non-coding RNA DsrA and Hfq does not require ribosome binding.

Authors:  Branislav Vecerek; Mads Beich-Frandsen; Armin Resch; Udo Bläsi
Journal:  Nucleic Acids Res       Date:  2009-12-06       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.