Literature DB >> 18687337

Adaptable molecular interactions guide phosphorylation of the SR protein ASF/SF2 by SRPK1.

Jonathan C Hagopian1, Chen-Ting Ma, Bryan R Meade, Claudio P Albuquerque, Jacky Chi Ki Ngo, Gourisankar Ghosh, Patricia A Jennings, Xiang-Dong Fu, Joseph A Adams.   

Abstract

The SR (arginine-serine rich) protein ASF/SF2 (also called human alternative splicing factor), an essential splicing factor, contains two functional modules consisting of tandem RNA recognition motifs (RRMs; RRM1-RRM2) and a C-terminal arginine-serine repeat region (RS domain, a domain rich in arginine-serine repeats). The SR-specific protein kinase (SRPK) 1 phosphorylates the RS domain at multiple serines using a directional (C-terminal-to-N-terminal) and processive mechanism--a process that directs the SR protein to the nucleus and influences protein-protein interactions associated with splicing function. To investigate how SRPK1 accomplishes this feat, the enzyme-substrate complex was analyzed using single-turnover and multiturnover kinetic methods. Deletion studies revealed that while recognition of the RS domain by a docking groove on SRPK1 is sufficient to initiate the processive and directional mechanism, continued processive phosphorylation in the presence of building repulsive charge relies on the fine-tuning of contacts with the RRM1-RRM2 module. An electropositive pocket in SRPK1 that stabilizes newly phosphorylated serines enhanced processive phosphorylation of later serines. These data indicate that SRPK1 uses stable, yet highly flexible protein-protein interactions to facilitate both early and late phases of the processive phosphorylation of SR proteins.

Entities:  

Mesh:

Substances:

Year:  2008        PMID: 18687337      PMCID: PMC2741138          DOI: 10.1016/j.jmb.2008.07.055

Source DB:  PubMed          Journal:  J Mol Biol        ISSN: 0022-2836            Impact factor:   5.469


  35 in total

Review 1.  Exonic splicing enhancers: mechanism of action, diversity and role in human genetic diseases.

Authors:  B J Blencowe
Journal:  Trends Biochem Sci       Date:  2000-03       Impact factor: 13.807

2.  A common phosphate binding site explains the unique substrate specificity of GSK3 and its inactivation by phosphorylation.

Authors:  S Frame; P Cohen; R M Biondi
Journal:  Mol Cell       Date:  2001-06       Impact factor: 17.970

Review 3.  Sorting out the complexity of SR protein functions.

Authors:  B R Graveley
Journal:  RNA       Date:  2000-09       Impact factor: 4.942

4.  Crystal structure of glycogen synthase kinase 3 beta: structural basis for phosphate-primed substrate specificity and autoinhibition.

Authors:  R Dajani; E Fraser; S M Roe; N Young; V Good; T C Dale; L H Pearl
Journal:  Cell       Date:  2001-06-15       Impact factor: 41.582

5.  Regulated cellular partitioning of SR protein-specific kinases in mammalian cells.

Authors:  Jian-Hua Ding; Xiang-Yang Zhong; Jonathan C Hagopian; Marissa M Cruz; Gourisankar Ghosh; James Feramisco; Joseph A Adams; Xiang-Dong Fu
Journal:  Mol Biol Cell       Date:  2005-11-30       Impact factor: 4.138

6.  Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.

Authors:  H X Liu; S L Chew; L Cartegni; M Q Zhang; A R Krainer
Journal:  Mol Cell Biol       Date:  2000-02       Impact factor: 4.272

7.  Ordered multi-site phosphorylation of the splicing factor ASF/SF2 by SRPK1.

Authors:  Chen-Ting Ma; Adolfo Velazquez-Dones; Jonathan C Hagopian; Gourisankar Ghosh; Xiang-Dong Fu; Joseph A Adams
Journal:  J Mol Biol       Date:  2007-08-21       Impact factor: 5.469

8.  A sliding docking interaction is essential for sequential and processive phosphorylation of an SR protein by SRPK1.

Authors:  Jacky Chi Ki Ngo; Kayla Giang; Sutapa Chakrabarti; Chen-Ting Ma; Nhat Huynh; Jonathan C Hagopian; Pieter C Dorrestein; Xiang-Dong Fu; Joseph A Adams; Gourisankar Ghosh
Journal:  Mol Cell       Date:  2008-03-14       Impact factor: 17.970

Review 9.  Unbalanced alternative splicing and its significance in cancer.

Authors:  Julian P Venables
Journal:  Bioessays       Date:  2006-04       Impact factor: 4.345

10.  Conserved SR protein kinase functions in nuclear import and its action is counteracted by arginine methylation in Saccharomyces cerevisiae.

Authors:  C Y Yun; X D Fu
Journal:  J Cell Biol       Date:  2000-08-21       Impact factor: 10.539

View more
  27 in total

1.  Acetylation and phosphorylation of SRSF2 control cell fate decision in response to cisplatin.

Authors:  Valerie Edmond; Elodie Moysan; Saadi Khochbin; Patrick Matthias; Christian Brambilla; Elisabeth Brambilla; Sylvie Gazzeri; Beatrice Eymin
Journal:  EMBO J       Date:  2010-12-14       Impact factor: 11.598

2.  Regulation of SR protein phosphorylation and alternative splicing by modulating kinetic interactions of SRPK1 with molecular chaperones.

Authors:  Xiang-Yang Zhong; Jian-Hua Ding; Joseph A Adams; Gourisankar Ghosh; Xiang-Dong Fu
Journal:  Genes Dev       Date:  2009-02-15       Impact factor: 11.361

3.  Unique role of SRSF2 in transcription activation and diverse functions of the SR and hnRNP proteins in gene expression regulation.

Authors:  Sudong Mo; Xiong Ji; Xiang-Dong Fu
Journal:  Transcription       Date:  2013 Sep-Dec

Review 4.  The role of RNA alternative splicing in regulating cancer metabolism.

Authors:  Itamar Kozlovski; Zahava Siegfried; Adi Amar-Schwartz; Rotem Karni
Journal:  Hum Genet       Date:  2017-04-20       Impact factor: 4.132

5.  Aberrant transcriptional regulation could explain phenotypic variability in autosomal recessive polycystic kidney disease.

Authors:  Friedrich C Luft
Journal:  J Mol Med (Berl)       Date:  2014-10       Impact factor: 4.599

Review 6.  Phosphorylation mechanism and structure of serine-arginine protein kinases.

Authors:  Gourisankar Ghosh; Joseph A Adams
Journal:  FEBS J       Date:  2011-01-12       Impact factor: 5.542

7.  N-terminus of the protein kinase CLK1 induces SR protein hyperphosphorylation.

Authors:  Brandon E Aubol; Ryan M Plocinik; Malik M Keshwani; Maria L McGlone; Jonathan C Hagopian; Gourisankar Ghosh; Xiang-Dong Fu; Joseph A Adams
Journal:  Biochem J       Date:  2014-08-15       Impact factor: 3.857

Review 8.  Regulation of splicing by SR proteins and SR protein-specific kinases.

Authors:  Zhihong Zhou; Xiang-Dong Fu
Journal:  Chromosoma       Date:  2013-03-24       Impact factor: 4.316

9.  Allosteric interactions direct binding and phosphorylation of ASF/SF2 by SRPK1.

Authors:  Nhat Huynh; Chen-Ting Ma; Ngoc Giang; Jonathan Hagopian; Jacky Ngo; Joseph Adams; Gourisankar Ghosh
Journal:  Biochemistry       Date:  2009-12-08       Impact factor: 3.162

10.  Partitioning RS domain phosphorylation in an SR protein through the CLK and SRPK protein kinases.

Authors:  Brandon E Aubol; Ryan M Plocinik; Jonathan C Hagopian; Chen-Ting Ma; Maria L McGlone; Reeti Bandyopadhyay; Xiang-Dong Fu; Joseph A Adams
Journal:  J Mol Biol       Date:  2013-05-23       Impact factor: 5.469

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.