Literature DB >> 1868218

A quick method to estimate the T-DNA copy number in transgenic plants at an early stage after transformation, using inverse PCR.

M P Does1, B M Dekker, M J de Groot, R Offringa.   

Abstract

Agrobacterium-mediated transformation of plants is known to result in transgenic plants with a variable number of integrated T-DNA copies. Our aim was to obtain transgenic tobacco plants containing one integrated T-DNA copy per genome. Therefore, a quick method was developed to estimate the T-DNA copy number of young transgenic plantlets within 10 weeks after transformation. Inverse polymerase chain reaction (IPCR) was used to amplify junction fragments, i.e. plant genomic DNA sequences flanking the known T-DNA sequences.

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Year:  1991        PMID： 1868218     DOI： 10.1007/bf00036819

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  2 in total

1.  Studies of the structure and functional organization of foreign DNA integrated into the genome of Nicotiana tabacum.

Authors:  A P Czernilofsky; R Hain; B Baker; U Wirtz
Journal:  DNA       Date:  1986-12

2.  Extrachromosomal homologous recombination and gene targeting in plant cells after Agrobacterium mediated transformation.

Authors:  R Offringa; M J de Groot; H J Haagsman; M P Does; P J van den Elzen; P J Hooykaas
Journal:  EMBO J       Date:  1990-10       Impact factor: 11.598
  2 in total
  17 in total

1.  Molecular characterization of transgenic shallots (Allium cepa L.) by adaptor ligation PCR (AL-PCR) and sequencing of genomic DNA flanking T-DNA borders.

Authors:  S J Zheng; B Henken; E Sofiari; E Jacobsen; F A Krens; C Kik
Journal:  Transgenic Res       Date:  2001-06       Impact factor: 2.788

2.  Regulatory sequences of Arabidopsis drive reporter gene expression in nematode feeding structures.

Authors:  N Barthels; F M van der Lee; J Klap; O J Goddijn; M Karimi; P Puzio; F M Grundler; S A Ohl; K Lindsey; L Robertson; W M Robertson; M Van Montagu; G Gheysen; P C Sijmons
Journal:  Plant Cell       Date:  1997-12       Impact factor: 11.277

3.  Isolation and characterization of an auxin-inducible glutathione S-transferase gene of Arabidopsis thaliana.

Authors:  D A van der Kop; M Schuyer; B Scheres; B J van der Zaal; P J Hooykaas
Journal:  Plant Mol Biol       Date:  1996-02       Impact factor: 4.076

Review 4.  Plant-nematode interactions.

Authors:  P C Sijmons
Journal:  Plant Mol Biol       Date:  1993-12       Impact factor: 4.076

5.  Nonreciprocal homologous recombination between Agrobacterium transferred DNA and a plant chromosomal locus.

Authors:  R Offringa; M E Franke-van Dijk; M J De Groot; P J van den Elzen; P J Hooykaas
Journal:  Proc Natl Acad Sci U S A       Date:  1993-08-01       Impact factor: 11.205

6.  Reduction of ribulose biphosphate carboxylase activase levels in tobacco (Nicotiana tabacum) by antisense RNA reduces ribulose biphosphate carboxylase carbamylation and impairs photosynthesis.

Authors:  C J Mate; G S Hudson; S von Caemmerer; J R Evans; T J Andrews
Journal:  Plant Physiol       Date:  1993-08       Impact factor: 8.340

7.  Non-recombinant background in gene targeting: illegitimate recombination between a hpt gene and a defective 5' deleted nptII gene can restore a Kmr phenotype in tobacco.

Authors:  M J de Groot; R Offringa; J Groet; M P Does; P J Hooykaas; P J van den Elzen
Journal:  Plant Mol Biol       Date:  1994-07       Impact factor: 4.076

8.  Female reproductive tissues are the primary target of Agrobacterium-mediated transformation by the Arabidopsis floral-dip method.

Authors:  C Desfeux; S J Clough; A F Bent
Journal:  Plant Physiol       Date:  2000-07       Impact factor: 8.340

9.  Potassium and carrot embryogenesis: are K+ channels necessary for development?

Authors:  Alex Costa; Armando Carpaneto; Serena Varotto; Elide Formentin; Oriano Marin; Elisabetta Barizza; Mario Terzi; Franco Gambale; Fiorella Lo Schiavo
Journal:  Plant Mol Biol       Date:  2004-04       Impact factor: 4.076

10.  Production of transgenic lily plants by Agrobacterium-mediated transformation.

Authors:  Y Hoshi; M Kondo; S Mori; Y Adachi; M Nakano; H Kobayashi
Journal:  Plant Cell Rep       Date:  2003-09-04       Impact factor: 4.570
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