Literature DB >> 14685763

Production of transgenic lily plants by Agrobacterium-mediated transformation.

Y Hoshi1, M Kondo, S Mori, Y Adachi, M Nakano, H Kobayashi.   

Abstract

A system for the production of transgenic plants was developed for the Oriental hybrid lily, Lilium cv. Acapulco, by Agrobacterium-mediated genetic transformation. Filament-derived calli were co-cultivated with A. tumefaciens strain EHA101/pIG121Hm, which harbored a binary vector carrying the neomycin phosphotransferase II, hygromycin phosphotransferase, and intron-containing beta-glucuronidase genes in the T-DNA region. Six hygromycin-resistant (Hyg(r)) culture lines were obtained from 200 calli by scratching them with sandpaper prior to inoculation and using NH(4)NO(3)-free medium for co-cultivation and a hygromycin-containing regeneration medium for selection. Hyg(r) culture lines regenerated shoots, which developed into plantlets following transfer to a plant growth regulator-free medium. All of these plantlets were verified to be transgenic by GUS histochemical assay and inverse PCR analysis.

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Year:  2003        PMID： 14685763     DOI： 10.1007/s00299-003-0700-z

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  16 in total

1.  High-frequency T-DNA-mediated gene tagging in plants.

Authors:  C Koncz; N Martini; R Mayerhofer; Z Koncz-Kalman; H Körber; G P Redei; J Schell
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2.  Molecular characterization of transgenic shallots (Allium cepa L.) by adaptor ligation PCR (AL-PCR) and sequencing of genomic DNA flanking T-DNA borders.

Authors:  S J Zheng; B Henken; E Sofiari; E Jacobsen; F A Krens; C Kik
Journal:  Transgenic Res       Date:  2001-06       Impact factor: 2.788

3.  A quick method to estimate the T-DNA copy number in transgenic plants at an early stage after transformation, using inverse PCR.

Authors:  M P Does; B M Dekker; M J de Groot; R Offringa
Journal:  Plant Mol Biol       Date:  1991-07       Impact factor: 4.076

4.  Efficient transformation of papaya by coat protein gene of papaya ringspot virus mediated byAgrobacterium following liquid-phase wounding of embryogenic tissues with caborundum.

Authors:  Y H Cheng; J S Yang; S D Yeh
Journal:  Plant Cell Rep       Date:  1996-12       Impact factor: 4.570

5.  Gene identification with sequenced T-DNA tags generated by transformation of Arabidopsis cell suspension.

Authors:  J Mathur; L Szabados; S Schaefer; B Grunenberg; A Lossow; E Jonas-Straube; J Schell; C Koncz; Z Koncz-Kálmán
Journal:  Plant J       Date:  1998-03       Impact factor: 6.417

6.  A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences.

Authors:  T Triglia; M G Peterson; D J Kemp
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

7.  Genetic applications of an inverse polymerase chain reaction.

Authors:  H Ochman; A S Gerber; D L Hartl
Journal:  Genetics       Date:  1988-11       Impact factor: 4.562

8.  Optimizing the biolistic process for different biological applications.

Authors:  J C Sanford; F D Smith; J A Russell
Journal:  Methods Enzymol       Date:  1993       Impact factor: 1.600

9.  Transformation of sunflower (Helianthus annuus L.): a reliable protocol.

Authors:  N Knittel; V Gruber; G Hahne; P Lénée
Journal:  Plant Cell Rep       Date:  1994-12       Impact factor: 4.570

10.  Transformation of sunflower (Helianthus annuus L.) following wounding with glass beads.

Authors:  W S Grayburn; B A Vick
Journal:  Plant Cell Rep       Date:  1995-02       Impact factor: 4.570
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  7 in total

1.  Efficient genetic transformation and regeneration system from hairy root of Origanum vulgare.

Authors:  Peyman Habibi; Maria Fatima Grossi de Sa; André Luís Lopes da Silva; Abdullah Makhzoum; Jefferson da Luz Costa; Ivo Albertto Borghetti; Carlos Ricardo Soccol
Journal:  Physiol Mol Biol Plants       Date:  2016-04-30

Review 2.  Biotechnological advances in Lilium.

Authors:  Mehdi Bakhshaie; Solmaz Khosravi; Pejman Azadi; Hedayat Bagheri; Jaap M van Tuyl
Journal:  Plant Cell Rep       Date:  2016-06-18       Impact factor: 4.570

3.  Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants.

Authors:  Mei-Kuen Yip; Hsiang-En Huang; Mang-Jye Ger; Shih-Hua Chiu; Yuh-Chih Tsai; Chin-I Lin; Teng-Yung Feng
Journal:  Plant Cell Rep       Date:  2006-10-11       Impact factor: 4.570

4.  Acquirement of CRY8DB Transgenic Tall Fescue (Festuca arundinacea Schreb.) by Agrobacterium tumefaciens to Develop Resistance Against Pentodon idiota Herbest.

Authors:  Hamid Reza Hosseini; Hassan Salehi; Mahmood Alichi
Journal:  Mol Biotechnol       Date:  2019-07       Impact factor: 2.695

5.  Importance of co-cultivation medium pH for successful Agrobacterium-mediated transformation of Lilium x formolongi.

Authors:  M Ogaki; Y Furuichi; K Kuroda; D P Chin; Y Ogawa; M Mii
Journal:  Plant Cell Rep       Date:  2007-12-04       Impact factor: 4.570

6.  Elimination of macro elements from inoculation and co-cultivation media enhances the efficiency of Agrobacterium-mediated transformation in Petunia.

Authors:  Aso Nobakht Vakili; Hedayat Bagheri; Pejman Azadi
Journal:  Physiol Mol Biol Plants       Date:  2018-05-31

7.  Development of Efficient Protocols for Stable and Transient Gene Transformation for Wolffia Globosa Using Agrobacterium.

Authors:  P P M Heenatigala; Jingjing Yang; Anthony Bishopp; Zuoliang Sun; Gaojie Li; Sunjeet Kumar; Shiqi Hu; Zhigang Wu; Wei Lin; Lunguang Yao; Pengfei Duan; Hongwei Hou
Journal:  Front Chem       Date:  2018-06-21       Impact factor: 5.221
  7 in total

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