Literature DB >> 1867849

S1 nuclease transcript mapping using sequenase-derived single-stranded probes.

A D Sharrocks1, D P Hornby.   

Abstract

We report here a simple method for the production of high specific activity single-stranded DNA probes for S1 nuclease transcript mapping. Plasmid DNA is used as in vitro DNA templates for probe production by primer extension using Sequenase. This allows the production of long single-stranded probes without the need to subclone restriction fragments into M13 vectors. The mapping of the 5' ends of the phage P1 mod gene transcripts is shown as an example.

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Year:  1991        PMID： 1867849

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  5 in total

1.  The Streptomyces glaucescens TcmR protein represses transcription of the divergently oriented tcmR and tcmA genes by binding to an intergenic operator region.

Authors:  P G Guilfoile; C R Hutchinson
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490

2.  Sequence and transcriptional analysis of the Streptomyces glaucescens tcmAR tetracenomycin C resistance and repressor gene loci.

Authors:  P G Guilfoile; C R Hutchinson
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490

3.  Transcriptional analysis of the Streptomyces glaucescens tetracenomycin C biosynthesis gene cluster.

Authors:  H Decker; C R Hutchinson
Journal:  J Bacteriol       Date:  1993-06       Impact factor: 3.490

4.  A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin.

Authors:  P G Guilfoile; C R Hutchinson
Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-01       Impact factor: 11.205

5.  Functional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius.

Authors:  K Madduri; C R Hutchinson
Journal:  J Bacteriol       Date:  1995-07       Impact factor: 3.490
  5 in total

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