BACKGROUND: Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive. OBJECTIVE: To develop a one-step RT-PCR assay to detect ZIKV in human serum. STUDY DESIGN: An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts. RESULTS: The assay's detection limit and repeatability were respectively 7.7pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected. CONCLUSIONS: The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples.
BACKGROUND:Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive. OBJECTIVE: To develop a one-step RT-PCR assay to detect ZIKV in human serum. STUDY DESIGN: An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts. RESULTS: The assay's detection limit and repeatability were respectively 7.7pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected. CONCLUSIONS: The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples.
Authors: Michaela J Schultz; Sharon Isern; Scott F Michael; Ronald B Corley; John H Connor; Horacio M Frydman Journal: J Virol Date: 2017-06-26 Impact factor: 5.103
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Authors: Maria Lucia Brito Ferreira; Carlos Alexandre Antunes de Brito; Álvaro José Porto Moreira; Maria Íris de Morais Machado; Adélia Henriques-Souza; Marli Tenório Cordeiro; Ernesto Torres de Azevedo Marques; Lindomar José Pena Journal: Am J Trop Med Hyg Date: 2017-11 Impact factor: 2.345
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Authors: Demócrito de Barros Miranda-Filho; Celina Maria Turchi Martelli; Ricardo Arraes de Alencar Ximenes; Thalia Velho Barreto Araújo; Maria Angela Wanderley Rocha; Regina Coeli Ferreira Ramos; Rafael Dhalia; Rafael Freitas de Oliveira França; Ernesto Torres de Azevedo Marques Júnior; Laura Cunha Rodrigues Journal: Am J Public Health Date: 2016-04 Impact factor: 9.308