| Literature DB >> 18664586 |
Li Xu1, Helena Yee, Christina Chan, Arun K Kashyap, Lawrence Horowitz, Michael Horowitz, Ramesh R Bhatt, Richard A Lerner.
Abstract
A unique type of combinatorial protein libraries has been constructed. These libraries are based on the pre-B cell receptor (pre-BCR). The pre-BCR is a protein that is produced during normal development of the antibody repertoire. Unlike that of canonical antibodies, the pre-BCR subunit is a trimer that is composed of an antibody heavy chain paired with two surrogate light chain (SLC) components. Combinatorial libraries based on these pre-BCR proteins in which diverse heavy chains are paired with a fixed SLC were expressed in mammalian, Escherichia coli, and phagemid systems. These libraries contain members that have nanomolar affinity for antigen. We term this type of antigen-binding protein a "surrobody" to distinguish it from the canonical antibody molecule.Entities:
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Year: 2008 PMID： 18664586 PMCID： PMC2504838 DOI： 10.1073/pnas.0805293105
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205