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Literature DB >> 18661299

Partial characterization of Enterococcus faecalis bacteriophage F4.

K Nigutová¹, I Styriak, P Javorský, P Pristas.

Abstract

Large Enterococcus faecalis F4 bacteriophage (described earlier) consisting of double-stranded linear DNA of approximately 60 kb was characterized. Library was prepared of its random DNA fragments and selected recombinants were sequenced. Three phage essential genes were characterized: DNA polymerase, replicative DNA helicase and a minor capsid protein, showing only limited homology to other known phage encoded genes. The occurrence of these genes among enterococci was determined by PCR method. Only two out of 40 tested isolates possessed all three genes, another three isolates contained at least one of the genes, demonstrating low frequency F4 lysogens among natural enterococcal isolates.

Entities: Species

Mesh：

Substances：
Viral Proteins

Year: 2008 PMID： 18661299 DOI： 10.1007/s12223-008-0033-y

Source DB: PubMed Journal: Folia Microbiol (Praha) ISSN： 0015-5632 Impact factor: 2.099

8 in total

1. Lectin-like binding and antibiotic sensitivity of enterococci from wild herbivores.

Authors: Igor Styriak; Andrea Lauková; Asa Ljungh
Journal: Microbiol Res Date: 2002 Impact factor: 5.415

2. 16S ribosomal DNA amplification for phylogenetic study.

Authors: W G Weisburg; S M Barns; D A Pelletier; D J Lane
Journal: J Bacteriol Date: 1991-01 Impact factor: 3.490

Review 3. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

Authors: S F Altschul; T L Madden; A A Schäffer; J Zhang; Z Zhang; W Miller; D J Lipman
Journal: Nucleic Acids Res Date: 1997-09-01 Impact factor: 16.971

4. Lack of surface receptors not restriction-modification system determines F4 phage resistance in Streptococcus bovis II/1.

Authors: I Styriak; P Pristas; P Javorský
Journal: Folia Microbiol (Praha) Date: 1998 Impact factor: 2.099

5. Construction of an integration-proficient vector based on the site-specific recombination mechanism of enterococcal temperate phage phiFC1.

Authors: Hee-Youn Yang; Young-Woo Kim; Hyo-Ihl Chang
Journal: J Bacteriol Date: 2002-04 Impact factor: 3.490

6. Morphological diversity of ruminal bacteriophages from sheep and cattle.

Authors: A V Klieve; T Bauchop
Journal: Appl Environ Microbiol Date: 1988-06 Impact factor: 4.792

7. A versatile quick-prep of genomic DNA from gram-positive bacteria.

Authors: A Pospiech; B Neumann
Journal: Trends Genet Date: 1995-06 Impact factor: 11.639

8. Lysogenic bacteriophage M1 from Selenomonas ruminantium: isolation, characterization and DNA sequence analysis of the integration site.

Authors: Judy P E Cheong; John D Brooker
Journal: Microbiology (Reading) Date: 1998-08 Impact factor: 2.777

8 in total

5 in total

Partial characterization of Enterococcus faecalis bacteriophage F4.

1. Lectin-like binding and antibiotic sensitivity of enterococci from wild herbivores.

2. 16S ribosomal DNA amplification for phylogenetic study.

Review 3. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

4. Lack of surface receptors not restriction-modification system determines F4 phage resistance in Streptococcus bovis II/1.

5. Construction of an integration-proficient vector based on the site-specific recombination mechanism of enterococcal temperate phage phiFC1.

6. Morphological diversity of ruminal bacteriophages from sheep and cattle.

7. A versatile quick-prep of genomic DNA from gram-positive bacteria.

8. Lysogenic bacteriophage M1 from Selenomonas ruminantium: isolation, characterization and DNA sequence analysis of the integration site.

1. Novel Bacteriophages in Enterococcus spp.

2. Eradication of Enterococcus faecalis by phage therapy in chronic bacterial prostatitis--case report.

Review 3. The influence of external factors on bacteriophages--review.

4. Structural proteins of Enterococcus faecalis bacteriophage ϕEf11.

Review 5. Phage therapy against Enterococcus faecalis in dental root canals.