Henrik Endeman1, Bjorn L Herpers2, Ben A W de Jong2, G Paul Voorn2, Jan C Grutters3, Heleen van Velzen-Blad2, Douwe H Biesma4. 1. Department of Intensive Care Medicine, Onze Lieve Vrouwe Gasthuis, Amsterdam. Electronic address: henrik.endeman@planet.nl. 2. Medical Microbiology and Immunology, St. Antonius Ziekenhuis Nieuwegein, Nieuwegein, the Netherlands. 3. Departments of Pulmonary Medicine, St. Antonius Ziekenhuis Nieuwegein, Nieuwegein, the Netherlands. 4. Department of Internal Medicine, University Medical Center Utrecht, Utrecht.
Abstract
BACKGROUND: Community-acquired pneumonia (CAP) is most frequently caused by Streptococcus pneumoniae, Haemophilus influenzae, atypical pathogens, and respiratory viruses. Susceptibility to CAP can be increased by single-nucleotide polymorphisms (SNPs) within the mannose-binding lectin (MBL) gene. We questioned whether MBL polymorphisms are associated with the susceptibility to and outcome of CAP and its most common pathogens. METHODS: All adult patients presenting with CAP in a 23-month period were included in this study. Frequencies of SNPs were determined for the promoter X/Y and the three coding SNPs in exon 1 (A/0). Six genotypes were constructed representing patients with sufficient and deficient serum levels of MBL. The results of the patients with CAP were compared with control subjects. RESULTS: In 199 patients and 223 control subjects, MBL genotypes were determined. There were no differences in MBL genotype frequencies between patients with CAP in general, pneumonia caused by S pneumoniae or H influenzae, and control subjects. The frequency of sufficient MBL genotypes was nonsignificantly higher in patients with pneumonia with Legionella sp and Mycoplasma pneumoniae. In Legionella spp, the sufficient YA/YA genotype was significantly more frequent than in control subjects (odds ratio [OR], 5.43; confidence interval [CI], 1.32 to 22.41; p = 0.02). The frequency of the MBL-deficient genotype was significantly higher in patients with viral (co)infections (OR, 2.36; CI, 1.06 to 5.26; p = 0.03) and nonsignificantly higher in patients with pneumococcal pneumonia and viral (co)infections. MBL genotypes had no effect on outcome. CONCLUSIONS: MBL genotypes play a limited role in pneumococcal pneumonia. Sufficient MBL genotypes were more frequently found in a small group of patients with atypical pneumonia, and MBL-deficient genotypes were more frequently found in patients with viral (co)infections.
BACKGROUND: Community-acquired pneumonia (CAP) is most frequently caused by Streptococcus pneumoniae, Haemophilus influenzae, atypical pathogens, and respiratory viruses. Susceptibility to CAP can be increased by single-nucleotide polymorphisms (SNPs) within the mannose-binding lectin (MBL) gene. We questioned whether MBL polymorphisms are associated with the susceptibility to and outcome of CAP and its most common pathogens. METHODS: All adult patients presenting with CAP in a 23-month period were included in this study. Frequencies of SNPs were determined for the promoter X/Y and the three coding SNPs in exon 1 (A/0). Six genotypes were constructed representing patients with sufficient and deficient serum levels of MBL. The results of the patients with CAP were compared with control subjects. RESULTS: In 199 patients and 223 control subjects, MBL genotypes were determined. There were no differences in MBL genotype frequencies between patients with CAP in general, pneumonia caused by S pneumoniae or H influenzae, and control subjects. The frequency of sufficient MBL genotypes was nonsignificantly higher in patients with pneumonia with Legionella sp and Mycoplasma pneumoniae. In Legionella spp, the sufficient YA/YA genotype was significantly more frequent than in control subjects (odds ratio [OR], 5.43; confidence interval [CI], 1.32 to 22.41; p = 0.02). The frequency of the MBL-deficient genotype was significantly higher in patients with viral (co)infections (OR, 2.36; CI, 1.06 to 5.26; p = 0.03) and nonsignificantly higher in patients with pneumococcal pneumonia and viral (co)infections. MBL genotypes had no effect on outcome. CONCLUSIONS:MBL genotypes play a limited role in pneumococcal pneumonia. Sufficient MBL genotypes were more frequently found in a small group of patients with atypical pneumonia, and MBL-deficient genotypes were more frequently found in patients with viral (co)infections.
Authors: Barry B Mook-Kanamori; Madelijn Geldhoff; Tom van der Poll; Diederik van de Beek Journal: Clin Microbiol Rev Date: 2011-07 Impact factor: 26.132
Authors: Minke A E Rab; Aafke Meerveld-Eggink; Heleen van Velzen-Blad; Douwe van Loon; Ger T Rijkers; Okke de Weerdt Journal: Int J Hematol Date: 2017-09-26 Impact factor: 2.490
Authors: D A van Kessel; T W Hoffman; H van Velzen-Blad; P Zanen; G T Rijkers; J C Grutters Journal: Clin Exp Immunol Date: 2014-07 Impact factor: 4.330
Authors: B L Herpers; H Endeman; B A W de Jong; B M de Jongh; J C Grutters; D H Biesma; H van Velzen-Blad Journal: Clin Exp Immunol Date: 2009-06 Impact factor: 4.330
Authors: Lyubov E Salnikova; Tamara V Smelaya; Arkadiy M Golubev; Alexander V Rubanovich; Viktor V Moroz Journal: Mol Biol Rep Date: 2013-09-26 Impact factor: 2.316
Authors: Stephen J Chapman; Fredrik O Vannberg; Chiea C Khor; Anna Rautanen; Nicholas A Maskell; Christopher W H Davies; Catrin E Moore; Nicholas P Day; Derrick W Crook; Robert J O Davies; Adrian V S Hill Journal: BMC Med Genet Date: 2010-01-15 Impact factor: 2.103