| Literature DB >> 1863594 |
J Lankelma1, H S Mülder, F van Mourik, H W Wong Fong Sang, R Kraayenhof, R van Grondelle.
Abstract
Multidrug resistant (MDR) 2780AD human ovarian carcinoma cells were loaded with the fluorescent anticancer agent daunomycin (DN). Fluorescence anisotropy was lower than for corresponding A2780 wild-type cells, indicating that DN was less rigidly bound than in the wild-type cells. Average fluorescence quenching of DN was lower for 2780AD cells. Data were fitted into a model with a highly quenched fraction (fraction A), corresponding to DN intercalated in DNA, and an unquenched fraction (fraction B). The ratio A/B was one order of magnitude lower for the MDR cells than for the wild-type cells. Two other MDR cell lines were investigated and low A/B ratios were found in both cases. Thus, evidence has been provided that in MDR cells the DNA-bound fraction is relatively low and that more free DN is present, for example in acidic vesicles.Entities:
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Year: 1991 PMID： 1863594 DOI： 10.1016/0167-4889(91)90116-f
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002