BACKGROUND: The steady-state redox potential of the cysteine/cystine couple in human plasma provides a measure of oxidative stress, yet available assays are limited by either specificity or speed of assay. METHOD: The present study evaluated the use of LC-FTMS for identification based on accurate mass combined with quantification by stable isotopic dilution to rapidly determine cysteine and cystine concentration and cysteine/cystine steady-state redox potential in human plasma. RESULTS: A simple extraction procedure followed by a rapid LC separation eluted cysteine in 4 min and cystine in 1.5 min with simultaneous measurement of glutathione (GSH) and glutathione disulfide (GSSG). A study of five young (mean age=25.7) subjects and 5 older (mean age=67.8 y) subjects showed an increased oxidation with age. CONCLUSIONS: The analysis by LC-FTMS is suitable for high-throughput analysis of plasma cysteine, cystine and cysteine/cystine steady-state redox potential as clinical measures of oxidative stress.
BACKGROUND: The steady-state redox potential of the cysteine/cystine couple in human plasma provides a measure of oxidative stress, yet available assays are limited by either specificity or speed of assay. METHOD: The present study evaluated the use of LC-FTMS for identification based on accurate mass combined with quantification by stable isotopic dilution to rapidly determine cysteine and cystine concentration and cysteine/cystine steady-state redox potential in human plasma. RESULTS: A simple extraction procedure followed by a rapid LC separation eluted cysteine in 4 min and cystine in 1.5 min with simultaneous measurement of glutathione (GSH) and glutathione disulfide (GSSG). A study of five young (mean age=25.7) subjects and 5 older (mean age=67.8 y) subjects showed an increased oxidation with age. CONCLUSIONS: The analysis by LC-FTMS is suitable for high-throughput analysis of plasma cysteine, cystine and cysteine/cystine steady-state redox potential as clinical measures of oxidative stress.
Authors: Thomas D Nolin; M Elizabeth McMenamin; Jonathan Himmelfarb Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2007-02-21 Impact factor: 3.205
Authors: Dean P Jones; Vino C Mody; Joanne L Carlson; Michael J Lynn; Paul Sternberg Journal: Free Radic Biol Med Date: 2002-11-01 Impact factor: 7.376
Authors: Kristina A Kigerl; Daniel P Ankeny; Sanjay K Garg; Ping Wei; Zhen Guan; Wenmin Lai; Dana M McTigue; Ruma Banerjee; Phillip G Popovich Journal: Exp Neurol Date: 2011-11-04 Impact factor: 5.330
Authors: Young-Mi Go; Douglas I Walker; Yongliang Liang; Karan Uppal; Quinlyn A Soltow; ViLinh Tran; Frederick Strobel; Arshed A Quyyumi; Thomas R Ziegler; Kurt D Pennell; Gary W Miller; Dean P Jones Journal: Toxicol Sci Date: 2015-09-09 Impact factor: 4.849
Authors: Sushma K Cribbs; Youngja Park; David M Guidot; Greg S Martin; Lou Ann Brown; Jeffrey Lennox; Dean P Jones Journal: AIDS Res Hum Retroviruses Date: 2014-02-10 Impact factor: 2.205
Authors: Jolyn Fernandes; Joshua D Chandler; Ken H Liu; Karan Uppal; Young-Mi Go; Dean P Jones Journal: Food Chem Toxicol Date: 2018-04-21 Impact factor: 6.023
Authors: Jung H Suh; Robert Kim; Burcu Yavuz; Daniel Lee; Ashutosh Lal; Bruce N Ames; Mark K Shigenaga Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2009-07-02 Impact factor: 3.205
Authors: Quinlyn A Soltow; Frederick H Strobel; Keith G Mansfield; Lynn Wachtman; Youngja Park; Dean P Jones Journal: Metabolomics Date: 2013-03 Impact factor: 4.290