Literature DB >> 18627764

Automated measurement of the static light scattering of macromolecular solutions over a broad range of concentrations.

Cristina Fernández1, Allen P Minton.   

Abstract

A method and apparatus for automated measurement of the concentration dependence of static light scattering of protein solutions over a broad range of concentrations is described. The gradient of protein concentrations is created by successive dilutions of an initially concentrated solution contained within the scattering measurement cell, which is maintained at constant total volume. The method is validated by measurement of the concentration dependence of light scattering of bovine serum albumin, ovalbumin, and ovomucoid at concentrations up to 130 g/L. The experimentally obtained concentration dependence of scattering obtained from all three proteins is quantitatively consistent with the assumption that no significant self-association occurs over the measured range of concentrations.

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Year:  2008        PMID： 18627764      PMCID： PMC2577915          DOI： 10.1016/j.ab.2008.06.032

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  18 in total

1.  The specificities of chicken ovomucoid and ovoinhibitor.

Authors:  R E FEENEY; F C STEVENS; D T OSUGA
Journal:  J Biol Chem       Date:  1963-04       Impact factor: 5.157

2.  Refractive index of concentrated protein solutions.

Authors:  R BARER; S TKACZYK
Journal:  Nature       Date:  1954-05-01       Impact factor: 49.962

Review 3.  Challenges in the development of high protein concentration formulations.

Authors:  Steven J Shire; Zahra Shahrokh; Jun Liu
Journal:  J Pharm Sci       Date:  2004-06       Impact factor: 3.534

4.  Hidden self-association of proteins.

Authors:  N Muramatsu; A P Minton
Journal:  J Mol Recognit       Date:  1989-04       Impact factor: 2.137

5.  Rapid quantitative characterization of protein interactions by composition gradient static light scattering.

Authors:  Keiichi Kameyama; Allen P Minton
Journal:  Biophys J       Date:  2005-12-30       Impact factor: 4.033

6.  New methods for measuring macromolecular interactions in solution via static light scattering: basic methodology and application to nonassociating and self-associating proteins.

Authors:  Arun K Attri; Allen P Minton
Journal:  Anal Biochem       Date:  2005-02-01       Impact factor: 3.365

7.  How can biochemical reactions within cells differ from those in test tubes?

Authors:  Allen P Minton
Journal:  J Cell Sci       Date:  2006-07-15       Impact factor: 5.285

8.  Structural basis of eye lens transparency: light scattering by concentrated solutions of bovine alpha-crystallin proteins.

Authors:  J Z Xia; Q Wang; S Tatarkova; T Aerts; J Clauwaert
Journal:  Biophys J       Date:  1996-11       Impact factor: 4.033

9.  Analysis of non-ideal behavior in concentrated hemoglobin solutions.

Authors:  P D Ross; A P Minton
Journal:  J Mol Biol       Date:  1977-05-25       Impact factor: 5.469

10.  The effect of volume occupancy upon the thermodynamic activity of proteins: some biochemical consequences.

Authors:  A P Minton
Journal:  Mol Cell Biochem       Date:  1983       Impact factor: 3.396
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  8 in total

1.  Static light scattering from concentrated protein solutions II: experimental test of theory for protein mixtures and weakly self-associating proteins.

Authors:  Cristina Fernández; Allen P Minton
Journal:  Biophys J       Date:  2009-03-04       Impact factor: 4.033

2.  Reexamining protein-protein and protein-solvent interactions from Kirkwood-Buff analysis of light scattering in multi-component solutions.

Authors:  Marco A Blanco; Erinc Sahin; Yi Li; Christopher J Roberts
Journal:  J Chem Phys       Date:  2011-06-14       Impact factor: 3.488

3.  Effect of nonadditive repulsive intermolecular interactions on the light scattering of concentrated protein-osmolyte mixtures.

Authors:  Cristina Fernández; Allen P Minton
Journal:  J Phys Chem B       Date:  2010-12-22       Impact factor: 2.991

4.  Quantitative characterization of the concentration-dependent interaction between molecules of Dextran 70 in aqueous solution: Measurement and analysis in the context of thermodynamic and compressible sphere models.

Authors:  Cristina Fernández; Adedayo A Fodeke; Allen P Minton
Journal:  Biopolymers       Date:  2019-05-06       Impact factor: 2.505

5.  Mg(2+)-linked self-assembly of FtsZ in the presence of GTP or a GTP analogue involves the concerted formation of a narrow size distribution of oligomeric species.

Authors:  Begoña Monterroso; Rubén Ahijado-Guzmán; Belén Reija; Carlos Alfonso; Silvia Zorrilla; Allen P Minton; Germán Rivas
Journal:  Biochemistry       Date:  2012-05-22       Impact factor: 3.162

6.  Light-scattering-based analysis of biomolecular interactions.

Authors:  Daniel Some
Journal:  Biophys Rev       Date:  2013-03-06

7.  Control by potassium of the size distribution of Escherichia coli FtsZ polymers is independent of GTPase activity.

Authors:  Rubén Ahijado-Guzmán; Carlos Alfonso; Belén Reija; Estefanía Salvarelli; Jesús Mingorance; Silvia Zorrilla; Begoña Monterroso; Germán Rivas
Journal:  J Biol Chem       Date:  2013-08-12       Impact factor: 5.157

8.  Protein-protein interactions in dilute to concentrated solutions: α-chymotrypsinogen in acidic conditions.

Authors:  Marco A Blanco; Tatiana Perevozchikova; Vincenzo Martorana; Mauro Manno; Christopher J Roberts
Journal:  J Phys Chem B       Date:  2014-05-21       Impact factor: 2.991
  8 in total

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