Literature DB >> 1862522

A non-radioactive receptor assay for snake venom postsynaptic neurotoxins.

B G Stiles1.   

Abstract

A non-radioactive assay was developed for detecting the binding of postsynaptic neurotoxins to acetylcholine receptor (AchR) from Torpedo californica. Enzyme linked immunosorbent assay (ELISA) wells coated with long or short chain neurotoxins specifically bound to purified AchR while crotamine or two different cardiotoxins did not. Bound receptor was detected by antibody against AchR. Specificity was determined by dose-response experiments and competition studies using carbamylcholine chloride, acetylcholine chloride, or Naja naja atra cobrotoxin mixed with receptor.

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Year:  1991        PMID： 1862522     DOI： 10.1016/0041-0101(91)90024-l

Source DB:  PubMed          Journal:  Toxicon        ISSN： 0041-0101            Impact factor:   3.033


  4 in total

1.  Characterization of alpha-conotoxin interactions with the nicotinic acetylcholine receptor and monoclonal antibodies.

Authors:  J D Ashcom; B G Stiles
Journal:  Biochem J       Date:  1997-11-15       Impact factor: 3.857

2.  Characterization of monoclonal antibodies against Naja naja oxiana neurotoxin I.

Authors:  B G Stiles; F W Sexton; S B Guest; M A Olson; D C Hack
Journal:  Biochem J       Date:  1994-10-01       Impact factor: 3.857

3.  A novel in vitro potency assay of antisera against Thai Naja kaouthia based on nicotinic acetylcholine receptor binding.

Authors:  Kavi Ratanabanangkoon; Pavinee Simsiriwong; Kritsada Pruksaphon; Kae Yi Tan; Sukanya Eursakun; Choo Hock Tan; Bunkuea Chantrathonkul; Wongsakorn Wongwadhunyoo; Sirida Youngchim; Nget Hong Tan
Journal:  Sci Rep       Date:  2017-08-17       Impact factor: 4.379

4.  In Vitro Tests for Assessing the Neutralizing Ability of Snake Antivenoms: Toward the 3Rs Principles.

Authors:  José María Gutiérrez; Mariángela Vargas; Álvaro Segura; María Herrera; Mauren Villalta; Gabriela Solano; Andrés Sánchez; Cristina Herrera; Guillermo León
Journal:  Front Immunol       Date:  2021-01-11       Impact factor: 7.561
  4 in total

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