| Literature DB >> 18621079 |
Nobutaka Horie1, Anne-Lise Maag, Scott A Hamilton, Hideo Shichinohe, Tonya M Bliss, Gary K Steinberg.
Abstract
Intracerebral injection of the vasoconstrictor peptide, endothelin-1 (ET-1), has been used as a method to induce focal ischemia in rats. The relative technical simplicity of this model makes it attractive for use in mice. However, the effect of ET-1 on mouse brains has not been firmly established. In this study, we determined the ability of ET-1 to induce focal cerebral ischemia in four different mouse strains (CD1, C57/BL6, NOD/SCID, and FVB). In contrast to rats, intracerebral injection of ET-1 did not produce a lesion in any mouse strain tested. A combination of ET-1 injection with either CCA occlusion or N(G)-nitro-l-arginine methyl ester (l-NAME) injection produced only a small infarct and its size was strain-dependent. A triple combination of CCA occlusion with co-injection of ET-1 and l-NAME produced a lesion in all mouse strains tested, and this resulted in a significant motor deficit. However, lesion size was still relatively small and strain-dependent. This study shows that ET-1 has a much less potent effect for producing an infarct in mice than rats.Entities:
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Year: 2008 PMID： 18621079 PMCID： PMC2572560 DOI： 10.1016/j.jneumeth.2008.06.013
Source DB: PubMed Journal: J Neurosci Methods ISSN： 0165-0270 Impact factor: 2.390