| Literature DB >> 18615424 |
Noboru Okamura1, Taro Masuda, Akinobu Gotoh, Toshiro Shirakawa, Shuji Terao, Naoki Kaneko, Kazuki Suganuma, Makoto Watanabe, Toshiya Matsubara, Ryota Seto, Jun Matsumoto, Megumi Kawakami, Motohiro Yamamori, Tsutomu Nakamura, Tatsurou Yagami, Toshiyuki Sakaeda, Masato Fujisawa, Osamu Nishimura, Katsuhiko Okumura.
Abstract
Renal cell carcinoma (RCC) is relatively resistant to chemotherapy and radiotherapy. Recent advances in drug development are providing novel agents for the treatment of RCC, but the effects are still minimal. In addition, there is an urgent need to identify diagnostic markers for RCC. In this report, to discover potential diagnostic markers and therapeutic targets, we subjected RCC samples to a quantitative proteomic analysis utilizing 2-nitrobenzenesulfenyl (NBS) reagent. Proteins were extracted from RCC and adjacent normal tissue, obtained surgically from patients, and labeled with NBS reagent containing six (12)C or (13)C. This was followed by trypsin digestion and the enrichment of labeled peptides. Samples were then subjected to analysis by MALDI-TOF MS. NBS-labeled peptides with a 6 Da difference were identified by MS/MS. Thirty-four proteins were upregulated in more than 60% of the patients of which some were previously known, and some were novel. The identity of a few proteins was confirmed by Western blotting and quantitative real time RT-PCR. The results suggest that NBS-based quantitative proteomic analysis is useful for discovering diagnostic markers and therapeutic targets for RCC.Entities:
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Year: 2008 PMID： 18615424 DOI： 10.1002/pmic.200700619
Source DB: PubMed Journal: Proteomics ISSN： 1615-9853 Impact factor: 3.984