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Literature DB >> 1860823

The Actinobacillus pleuropneumoniae hemolysin determinant: unlinked appCA and appBD loci flanked by pseudogenes.

Abstract

The appBD genes encoding the secretion functions for the 110-kDa RTX hemolysin of Actinobacillus pleuropneumoniae have been cloned and sequenced. Unlike analogous genes from other RTX determinants, the appBD genes do not lie immediately downstream from the hemolysin structural gene, appA. Although isolated from a diverse group of gram-negative organisms, the appBD genes and the characterized RTX BD genes from other organisms all exhibit a high degree of homology at both the DNA and predicted amino acid sequence levels. Analysis of the DNA sequences 3' to appA and 5' to appB suggests that these regions harbor remnant RTX B and A pseudogenes, respectively. Although the appA gene is most similar to the lktA gene from Pasteurella haemolytica (Y. F. Chang, R. Young, and D. K. Struck, DNA 8:635-647, 1989), the RTX A pseudogene upstream from appB most closely resembles the hlyB gene from Escherichia coli, suggesting that the appCA and appBD operons were derived from different ancestral RTX determinants.

Entities: Gene Species

Mesh：

Substances：

Year: 1991 PMID： 1860823 PMCID： PMC208207 DOI： 10.1128/jb.173.16.5151-5158.1991

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

30 in total

Review 1. Structure and function of haemolysin B,P-glycoprotein and other members of a novel family of membrane translocators.

Authors: M A Blight; I B Holland
Journal: Mol Microbiol Date: 1990-06 Impact factor: 3.501

2. Genetical and functional organisation of the Escherichia coli haemolysin determinant 2001.

Authors: N Mackman; J M Nicaud; L Gray; I B Holland
Journal: Mol Gen Genet Date: 1985

3. Regulation of expression of the Pasteurella haemolytica leukotoxin determinant.

Authors: C A Strathdee; R Y Lo
Journal: J Bacteriol Date: 1989-11 Impact factor: 3.490

4. Nucleotide sequence of the leukotoxin genes of Pasteurella haemolytica A1.

Authors: R Y Lo; C A Strathdee; P E Shewen
Journal: Infect Immun Date: 1987-09 Impact factor: 3.441

5. Comparison of the haemolysin secretion protein HlyB from Proteus vulgaris and Escherichia coli; site-directed mutagenesis causing impairment of export function.

Authors: V Koronakis; E Koronakis; C Hughes
Journal: Mol Gen Genet Date: 1988-08

6. DNA sequence of the Pasteurella haemolytica leukotoxin gene cluster.

Authors: S K Highlander; M Chidambaram; M J Engler; G M Weinstock
Journal: DNA Date: 1989 Jan-Feb

7. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

8. Cloning and characterization of a hemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae.

Authors: Y F Chang; R Young; D K Struck
Journal: DNA Date: 1989-11

Review 9. A novel C-terminal signal sequence targets Escherichia coli haemolysin directly to the medium.

Authors: L Gray; K Baker; B Kenny; N Mackman; R Haigh; I B Holland
Journal: J Cell Sci Suppl Date: 1989

10. Transcriptional organization of the Escherichia coli hemolysin genes.

Authors: R A Welch; S Pellett
Journal: J Bacteriol Date: 1988-04 Impact factor: 3.490

20 in total

1. Separable domains define target cell specificities of an RTX hemolysin from Actinobacillus pleuropneumoniae.

Authors: D R McWhinney; Y F Chang; R Young; D K Struck
Journal: J Bacteriol Date: 1992-01 Impact factor: 3.490

2. Isolation and molecular characterization of spontaneously occurring cytolysin-negative mutants of Actinobacillus pleuropneumoniae serotype 7.

Authors: C Anderson; A A Potter; G F Gerlach
Journal: Infect Immun Date: 1991-11 Impact factor: 3.441

The Actinobacillus pleuropneumoniae hemolysin determinant: unlinked appCA and appBD loci flanked by pseudogenes.

Review 1. Structure and function of haemolysin B,P-glycoprotein and other members of a novel family of membrane translocators.

2. Genetical and functional organisation of the Escherichia coli haemolysin determinant 2001.

3. Regulation of expression of the Pasteurella haemolytica leukotoxin determinant.

4. Nucleotide sequence of the leukotoxin genes of Pasteurella haemolytica A1.

5. Comparison of the haemolysin secretion protein HlyB from Proteus vulgaris and Escherichia coli; site-directed mutagenesis causing impairment of export function.

6. DNA sequence of the Pasteurella haemolytica leukotoxin gene cluster.

7. DNA sequencing with chain-terminating inhibitors.

8. Cloning and characterization of a hemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae.

Review 9. A novel C-terminal signal sequence targets Escherichia coli haemolysin directly to the medium.

10. Transcriptional organization of the Escherichia coli hemolysin genes.

1. Separable domains define target cell specificities of an RTX hemolysin from Actinobacillus pleuropneumoniae.

2. Isolation and molecular characterization of spontaneously occurring cytolysin-negative mutants of Actinobacillus pleuropneumoniae serotype 7.

3. New nucleotide sequence data on the EMBL File Server.

4. Topology analysis of the colicin V export protein CvaA in Escherichia coli.

5. Detection of RTX toxin genes in gram-negative bacteria with a set of specific probes.

6. Molecular characterization of an RTX toxin determinant from Actinobacillus suis.

7. Comparison of the cytolysin II genetic determinants of Actinobacillus pleuropneumoniae serotypes.

8. Transposon mutagenesis in Actinobacillus pleuropneumoniae with a Tn10 derivative.

9. Structural analysis of the Actinobacillus pleuropneumoniae-RTX-toxin I (ApxI) operon.

10. Cloning and characterization of the Actinobacillus pleuropneumoniae-RTX-toxin III (ApxIII) gene.