Evolutionary operation (EVOP) to optimize three-dimensional biological experiments.

Evolutionary operation (EVOP) is used as an efficient technique for optimization of three variable experimental parameters using two-level factorial designs with center points. For metabolic function like enzyme synthesis by microorganisms, small amounts of metal ions, surfactants, and vitamins act as inducers. The desired quantities of these chemicals are, however, species dependent and have to be found out or each microorganism. By employing the EVOP technique, the requirements of these chemicals have been optimized for the production of protease by Rhizopus oryzae (RO, IIT KGP).