Literature DB >> 18595043

Immobilization can improve the stability of hybridoma antibody productivity in serum-free media.

G M Lee1, B O Palsson.   

Abstract

Hybridoma cells (S3H5/gamma2bA2) were cultivated in spinner flasks with 1% serum media and serum-free media. Monoclonal antibody productivity was maintained in 1% serum media. However, cells in serum-free media showed a decrease in antibody productivity, and it completely disappeared in IMDM-based low protein medium. This loss of antibody productivity was not observed when the cells were immobilized in alginate beads. In fact, immobilization enhanced the specific MAb productivity.

Entities:  

Year:  1990        PMID： 18595043     DOI： 10.1002/bit.260361010

Source DB:  PubMed          Journal:  Biotechnol Bioeng        ISSN： 0006-3592            Impact factor:   4.530


  9 in total

1.  Enhancing monoclonal antibodies and hybridoma cell lines.

Authors:  S S Seaver
Journal:  Cytotechnology       Date:  1992       Impact factor: 2.058

2.  Long-term Continuous Production of Monoclonal Antibody by Hybridoma Cells Immobilized in a Fibrous-Bed Bioreactor.

Authors:  Hui Zhu; Shang-Tian Yang
Journal:  Cytotechnology       Date:  2004-01       Impact factor: 2.058

3.  The influence of dissolved oxygen tension on the metabolic activity of an immobilized hybridoma population.

Authors:  J Thömmes; J Gätgens; M Biselli; P W Runstadler; C Wandrey
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058

4.  Enhanced specific antibody productivity of calcium alginate-entrapped hybridoma is cell line-specific.

Authors:  G M Lee; S J Kim; B O Palsson
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

5.  Investigation of parameters affecting a fixed bed bioreactor process for recombinant cell lines.

Authors:  A J Racher; J B Griffiths
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058

6.  Improvement of the culture stability of non-anchorage-dependent animal cells grown in serum-free media through immobilization.

Authors:  I Lüdemann; R Pörtner; C Schaefer; K Schick; K Srámkova; K Reher; M Neumaier; F Franěk; H Märkl
Journal:  Cytotechnology       Date:  1995-06       Impact factor: 2.058

7.  Hybridoma cells in a protein-free medium within a composite gel perfusion bioreactor.

Authors:  B Q Shen; P F Greenfield; S Reid
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

8.  Plant protein hydrolysates support CHO-320 cells proliferation and recombinant IFN-gamma production in suspension and inside microcarriers in protein-free media.

Authors:  J S Ballez; J Mols; C Burteau; S N Agathos; Y J Schneider
Journal:  Cytotechnology       Date:  2004-03       Impact factor: 2.058

Review 9.  FoldX as Protein Engineering Tool: Better Than Random Based Approaches?

Authors:  Oliver Buß; Jens Rudat; Katrin Ochsenreither
Journal:  Comput Struct Biotechnol J       Date:  2018-02-03       Impact factor: 7.271
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.