Enhanced specific antibody productivity of calcium alginate-entrapped hybridoma is cell line-specific.

In order to determine whether the enhanced specific antibody productivity (qMAb) of calcium alginate-entrapped hybridoma is cell line-specific, calcium alginate-entrapped hybridomas (4A2 and DB9G8) were cultivated under the condition where we had previously observed significantly enhanced qMAb of calcium alginate-entrapped S3H5/gamma 2bA2 hybridoma. Unlike S3H5/gamma 2bA2 hybridoma, neither 4A2 nor DB9G8 hybridomas showed persistently enhanced qMAb when they were entrapped in calcium alginate beads. The enhanced qMAb of entrapped 4A2 and DB9G8 hybridomas, which was 2-3 times higher than the qMAb of free-suspended cells in a control experiment, was observed only during the early stage of the culture. During the early stage of the culture, the viable cell concentration decreased probably due to cell damage during the entrapment process. As cell growth resumed, the qMAb decreased to the similar level of qMAb of free-suspended cells within 5-7 days. Thus, we conclude that the enhanced qMAb of calcium alginate-entrapped hybridomas is cell line-specific.