Literature DB >> 18586972

Denaturing gradient gel electrophoresis as a tool to determine batch similarity of probiotic cultures of porcine cecal bacteria.

Michael E Hume1, Charles M Scanlan, Roger B Harvey, Kathleen Andrews, James D Snodgrass, Armen G Nalian, Alexandra Martynova-Van Kley, David J Nisbet.   

Abstract

A continuous-flow porcine cecal bacterial culture has been used experimentally as treatment against enterotoxigenic Escherichia coli in weanling pigs. Periodically, the cultures must be started from frozen stock. Our results indicate that denaturing gradient gel electrophoresis can be applied as an indirect indication of culture similarity for each new batch generated from frozen stock.

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Year:  2008        PMID： 18586972      PMCID： PMC2519268          DOI： 10.1128/AEM.02580-07

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  Integration of microbial ecology and statistics: a test to compare gene libraries.

Authors:  Patrick D Schloss; Bret R Larget; Jo Handelsman
Journal:  Appl Environ Microbiol       Date:  2004-09       Impact factor: 4.792

2.  Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.

Authors:  G Muyzer; E C de Waal; A G Uitterlinden
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792

3.  Presentation of postweaning Escherichia coli diarrhea in southern Ontario, prevalence of hemolytic E. coli serogroups involved, and their antimicrobial resistance patterns.

Authors:  Rocio Amezcua; Robert M Friendship; Catherine E Dewey; Carlton Gyles; John M Fairbrother
Journal:  Can J Vet Res       Date:  2002-04       Impact factor: 1.310

4.  Inhibition of in vitro Salmonella Typhimurium colonization in porcine cecal bacteria continuous-flow competitive exclusion culturest.

Authors:  M E Hume; D J Nisbet; S A Buckley; R L Ziprin; R C Anderson; L H Stanker
Journal:  J Food Prot       Date:  2001-01       Impact factor: 2.077

5.  Competitive exclusion treatment reduces the mortality and fecal shedding associated with enterotoxigenic Escherichia coli infection in nursery-raised neonatal pigs.

Authors:  K J Genovese; R C Anderson; R B Harvey; D J Nisbet
Journal:  Can J Vet Res       Date:  2000-10       Impact factor: 1.310

6.  The effect of probiotic LSP 122 on the control of post-weaning diarrhoea syndrome of piglets.

Authors:  S C Kyriakis; V K Tsiloyiannis; J Vlemmas; K Sarris; A C Tsinas; C Alexopoulos; L Jansegers
Journal:  Res Vet Sci       Date:  1999-12       Impact factor: 2.534

Review 7.  The European ban on growth-promoting antibiotics and emerging consequences for human and animal health.

Authors:  Mark Casewell; Christian Friis; Enric Marco; Paul McMullin; Ian Phillips
Journal:  J Antimicrob Chemother       Date:  2003-07-01       Impact factor: 5.790

8.  Effect of mixed cecal microflora maintained in continuous culture and of dietary lactose on Salmonella typhimurium colonization in broiler chicks.

Authors:  D J Nisbet; D E Corrier; J R DeLoach
Journal:  Avian Dis       Date:  1993 Apr-Jun       Impact factor: 1.577

9.  Poultry digestive microflora biodiversity as indicated by denaturing gradient gel electrophoresis.

Authors:  M E Hume; L F Kubena; T S Edrington; C J Donskey; R W Moore; S C Ricke; D J Nisbet
Journal:  Poult Sci       Date:  2003-07       Impact factor: 3.352

10.  Competitive exclusion of Salmonella from the gut of neonatal and weaned pigs.

Authors:  K J Genovese; R C Anderson; R B Harvey; T R Callaway; T L Poole; T S Edrington; P J Fedorka-Cray; D J Nisbet
Journal:  J Food Prot       Date:  2003-08       Impact factor: 2.077
  10 in total
  1 in total

1.  Establishment and characterization of a competitive exclusion bacterial culture derived from Nile tilapia (Oreochromis niloticus) gut microbiomes showing antibacterial activity against pathogenic Streptococcus agalactiae.

Authors:  Javier Fernando Melo-Bolívar; Ruth Yolanda Ruiz Pardo; Michael E Hume; David J Nisbet; Fernando Rodríguez-Villamizar; Juan F Alzate; Howard Junca; Luisa Marcela Villamil Díaz
Journal:  PLoS One       Date:  2019-05-03       Impact factor: 3.240
  1 in total

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