BACKGROUND: Numerous epidemiologic studies have demonstrated an allergy-protective effect of farm life early in childhood. It has been hypothesized that environmental exposure to microbes may contribute to this effect. Because of their small size and thereby their potential for deposition in lower airways of small children, bacterial spores may be candidates for such allergy-protective effects. OBJECTIVE: To investigate immune responses elicited by exposure to Bacillus spores in experimental settings. METHODS: Animal shed and mattress dusts were analyzed for bacteria and fungi by aerobic and anaerobic growth. Bacillus licheniformis, the most prominent microorganism found in these samples, was investigated with respect to spore specific stimulation of pattern recognition receptors, monocyte-derived dendritic cells and T(H)-cell polarization in vitro as well as to the prevention of asthma development in a mouse model of allergic asthma. RESULTS: In vitro, B. licheniformis spores activated a T(H)1 cytokine expression profile. In vivo application of these spores resulted in less spore-specific but long-lasting immune activation preventing eosinophilia and goblet cell hyperplasia; however, they provoked an influx of neutrophils in lung tissue of asthmatic mice. CONCLUSION: Bacterial spores may contribute to the allergy-protective properties of farming environments, but their persistence in the lung causes ongoing immune activation in mouse experiments.
BACKGROUND: Numerous epidemiologic studies have demonstrated an allergy-protective effect of farm life early in childhood. It has been hypothesized that environmental exposure to microbes may contribute to this effect. Because of their small size and thereby their potential for deposition in lower airways of small children, bacterial spores may be candidates for such allergy-protective effects. OBJECTIVE: To investigate immune responses elicited by exposure to Bacillus spores in experimental settings. METHODS: Animal shed and mattress dusts were analyzed for bacteria and fungi by aerobic and anaerobic growth. Bacillus licheniformis, the most prominent microorganism found in these samples, was investigated with respect to spore specific stimulation of pattern recognition receptors, monocyte-derived dendritic cells and T(H)-cell polarization in vitro as well as to the prevention of asthma development in a mouse model of allergic asthma. RESULTS: In vitro, B. licheniformis spores activated a T(H)1 cytokine expression profile. In vivo application of these spores resulted in less spore-specific but long-lasting immune activation preventing eosinophilia and goblet cell hyperplasia; however, they provoked an influx of neutrophils in lung tissue of asthmatic mice. CONCLUSION: Bacterial spores may contribute to the allergy-protective properties of farming environments, but their persistence in the lung causes ongoing immune activation in mouse experiments.
Authors: Tobias Janke; Karin Schwaiger; Markus Ege; Carmen Fahn; Erika von Mutius; Johann Bauer; Melanie Mayer Journal: Curr Microbiol Date: 2013-03-10 Impact factor: 2.188
Authors: Andreas Kyburz; Angela Fallegger; Xiaozhou Zhang; Aleksandra Altobelli; Mariela Artola-Boran; Timothy Borbet; Sabine Urban; Petra Paul; Christian Münz; Stefan Floess; Jochen Huehn; Timothy L Cover; Martin J Blaser; Christian Taube; Anne Müller Journal: J Allergy Clin Immunol Date: 2018-09-19 Impact factor: 10.793
Authors: Vivian H Tang; Barbara J Chang; Ambuja Srinivasan; Leslie T Mathaba; Gerald B Harnett; Geoffrey A Stewart Journal: Exp Appl Acarol Date: 2013-06-20 Impact factor: 2.132