Literature DB >> 18579241

Analysis of lolB gene sequence and its use in the development of a PCR assay for the detection of Vibrio cholerae.

P Lalitha1, M N Siti Suraiya, K L Lim, S Y Lee, A R Nur Haslindawaty, Y Y Chan, Asma Ismail, Zainul F Zainuddin, M Ravichandran.   

Abstract

A PCR assay has been developed based on a lolB (hemM) gene, which was found to be highly conserved among the Vibrio cholerae species but non-conserved among the other enteric bacteria. The lolB PCR detected all O1, O139 and non-O1/non-O139 serogroup and biotypes of V. cholerae. The analytical specificity of this assay was 100% while the analytical sensitivity was 10 pg/microL and 10(3) CFU/mL at DNA and bacterial level respectively. The diagnostic sensitivity and specificity was 98.5% and 100% respectively.

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Year:  2008        PMID： 18579241     DOI： 10.1016/j.mimet.2008.05.001

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  6 in total

1.  Electrochemical genosensor for specific detection of the food-borne pathogen, Vibrio cholerae.

Authors:  Kim-Fatt Low; Kritsanaporn Chuenrangsikul; Patsamon Rijiravanich; Werasak Surareungchai; Yean-Yean Chan
Journal:  World J Microbiol Biotechnol       Date:  2011-12-18       Impact factor: 3.312

2.  Molecular evidence of cholera outbreak caused by a toxigenic Vibrio cholerae O1 El tor variant strain in Kelantan, Malaysia.

Authors:  Geik Yong Ang; Choo Yee Yu; Kamarudin Balqis; Husni Tan Elina; Hussin Azura; Mat Hussin Hani; Chan Yean Yean
Journal:  J Clin Microbiol       Date:  2010-09-08       Impact factor: 5.948

3.  Simple Visualized Detection Method of Virulence-Associated Genes of Vibrio cholerae by Loop-Mediated Isothermal Amplification.

Authors:  Mengjie Xu; Huiyu Fu; Dailing Chen; Zehuai Shao; Jun Zhu; Walid Q Alali; Lanming Chen
Journal:  Front Microbiol       Date:  2019-12-20       Impact factor: 5.640

4.  Duplex On-Site Detection of Vibrio cholerae and Vibrio vulnificus by Recombinase Polymerase Amplification and Three-Segment Lateral Flow Strips.

Authors:  Pei Wang; Lei Liao; Chao Ma; Xue Zhang; Junwei Yu; Longyu Yi; Xin Liu; Hui Shen; Song Gao; Qunwei Lu
Journal:  Biosensors (Basel)       Date:  2021-05-12

Review 5.  Review of two decades of cholera diagnostics--how far have we really come?

Authors:  Michal H Dick; Martine Guillerm; Francis Moussy; Claire-Lise Chaignat
Journal:  PLoS Negl Trop Dis       Date:  2012-10-11

6.  Virulence, Resistance, and Genomic Fingerprint Traits of Vibrio cholerae Isolated from 12 Species of Aquatic Products in Shanghai, China.

Authors:  Huiyu Fu; Pan Yu; Weili Liang; Biao Kan; Xu Peng; Lanming Chen
Journal:  Microb Drug Resist       Date:  2020-11-06       Impact factor: 3.431
  6 in total

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