Literature DB >> 18571198

Overexpression of phage HK022 Nun protein is toxic for Escherichia coli.

Augusto Uc-Mass1, Arkady Khodursky, Lewis Brown, Max E Gottesman.   

Abstract

The Nun protein of coliphage HK022 excludes superinfecting lambda phage. Nun recognizes and binds to the N utilization (nut) sites on phage lambda nascent RNA and induces transcription termination. Overexpression of Nun from a high-copy plasmid is toxic for Escherichia coli, despite the fact that nut sites are not encoded in the E. coli genome. Cells expressing Nun cannot exit stationary phase. Toxicity is related to transcription termination, since host and nun mutations that block termination also suppress cell killing. Nun inhibits expression of wild-type lacZ, but not lacZ expressed from the Crp/cAMP-independent lacUV5 promoter. Microarray and proteomic analyses show that Nun down-regulates crp and tnaA. Crp overexpression and high indole concentrations partially reverse Nun-mediated toxicity and restore lacZ expression.

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Year:  2008        PMID： 18571198      PMCID： PMC2597491          DOI： 10.1016/j.jmb.2008.05.030

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  27 in total

1.  The remarkable specificity of a new transcription termination factor suggests that the mechanisms of termination and antitermination are similar.

Authors:  J Robert; S B Sloan; R A Weisberg; M E Gottesman; R Robledo; D Harbrecht
Journal:  Cell       Date:  1987-11-06       Impact factor: 41.582

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Authors:  R L Gourse; H A de Boer; M Nomura
Journal:  Cell       Date:  1986-01-17       Impact factor: 41.582

3.  Constitutive expression of a transcription termination factor by a repressed prophage: promoters for transcribing the phage HK022 nun gene.

Authors:  R A King; P L Madsen; R A Weisberg
Journal:  J Bacteriol       Date:  2000-01       Impact factor: 3.490

4.  Indole can act as an extracellular signal in Escherichia coli.

Authors:  D Wang; X Ding; P N Rather
Journal:  J Bacteriol       Date:  2001-07       Impact factor: 3.490

Review 5.  Mechanisms for redox control of gene expression.

Authors:  C E Bauer; S Elsen; T H Bird
Journal:  Annu Rev Microbiol       Date:  1999       Impact factor: 15.500

6.  Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.

Authors:  L M Guzman; D Belin; M J Carson; J Beckwith
Journal:  J Bacteriol       Date:  1995-07       Impact factor: 3.490

7.  Interaction of the sigma factor and the nusA gene protein of E. coli with RNA polymerase in the initiation-termination cycle of transcription.

Authors:  J Greenblatt; J Li
Journal:  Cell       Date:  1981-05       Impact factor: 41.582

8.  Isolation of the gene encoding the Hin recombinational enhancer binding protein.

Authors:  R C Johnson; C A Ball; D Pfeffer; M I Simon
Journal:  Proc Natl Acad Sci U S A       Date:  1988-05       Impact factor: 11.205

9.  Escherichia coli mutations that block transcription termination by phage HK022 Nun protein.

Authors:  R Robledo; B L Atkinson; M E Gottesman
Journal:  J Mol Biol       Date:  1991-08-05       Impact factor: 5.469

10.  E.coli Fis protein activates ribosomal RNA transcription in vitro and in vivo.

Authors:  W Ross; J F Thompson; J T Newlands; R L Gourse
Journal:  EMBO J       Date:  1990-11       Impact factor: 11.598
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  7 in total

Review 1.  Transcription elongation.

Authors:  Arkady Mustaev; Jeffrey Roberts; Max Gottesman
Journal:  Transcription       Date:  2017-02-08

2.  Probing the structure of Nun transcription arrest factor bound to RNA polymerase.

Authors:  Arkady Mustaev; Christal L Vitiello; Max E Gottesman
Journal:  Proc Natl Acad Sci U S A       Date:  2016-07-19       Impact factor: 11.205

3.  HK022 Nun Requires Arginine-Rich Motif Residues Distinct from λ N.

Authors:  Caroline S Tawk; Ingrid R Ghattas; Colin A Smith
Journal:  J Bacteriol       Date:  2015-09-08       Impact factor: 3.490

4.  Glycine-rich RNA binding protein of Oryza sativa inhibits growth of M15 E. coli cells.

Authors:  Upasana Singh; Debadutta Deb; Amanjot Singh; Anil Grover
Journal:  BMC Res Notes       Date:  2011-01-26

Review 5.  Regulation of transcription elongation and termination.

Authors:  Robert S Washburn; Max E Gottesman
Journal:  Biomolecules       Date:  2015-05-29

6.  Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex.

Authors:  Jin Young Kang; Paul Dominic B Olinares; James Chen; Elizabeth A Campbell; Arkady Mustaev; Brian T Chait; Max E Gottesman; Seth A Darst
Journal:  Elife       Date:  2017-03-20       Impact factor: 8.140

7.  Bacteriophage HK022 Nun protein arrests transcription by blocking lateral mobility of RNA polymerase during transcription elongation.

Authors:  Christal L Vitiello; Max E Gottesman
Journal:  Bacteriophage       Date:  2014-07-30
  7 in total

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