OBJECTIVE: The purpose of this study was to determine the effects of enamel matrix derivative on mRNA expression of markers related to periodontal healing. STUDY DESIGN: Murine osteoprogenitor cells (MC3T3-E1) were grown for 12 and 16 days in mineralization media and stimulated with 100 microg/mL Emdogain (EMD). Cell cultures treated with 2% and 10% fetal calf serum (FCS) served as control. The mRNA expression of bone sialoprotein (BSP), osteopontin (OPN), and runt-related protein 2 (Runx2) was analyzed by real-time polymerase chain reaction. One-way analysis of variance was used for statistical analysis. RESULTS: Stimulation with EMD significantly (P < .01) enhanced mRNA expression of BSP up to 13.9-fold and of OPN up to 3.2-fold at day 16 compared with the 2% FCS control. The expression of mRNA for transcription factor Runx2 was not significantly changed. CONCLUSION: The beneficial effects seen in periodontal regeneration after treatment with EMD may be related to an increase of the mineralization markers BSP and OPN at mRNA level.
OBJECTIVE: The purpose of this study was to determine the effects of enamel matrix derivative on mRNA expression of markers related to periodontal healing. STUDY DESIGN:Murine osteoprogenitor cells (MC3T3-E1) were grown for 12 and 16 days in mineralization media and stimulated with 100 microg/mL Emdogain (EMD). Cell cultures treated with 2% and 10% fetal calf serum (FCS) served as control. The mRNA expression of bone sialoprotein (BSP), osteopontin (OPN), and runt-related protein 2 (Runx2) was analyzed by real-time polymerase chain reaction. One-way analysis of variance was used for statistical analysis. RESULTS: Stimulation with EMD significantly (P < .01) enhanced mRNA expression of BSP up to 13.9-fold and of OPN up to 3.2-fold at day 16 compared with the 2% FCS control. The expression of mRNA for transcription factor Runx2 was not significantly changed. CONCLUSION: The beneficial effects seen in periodontal regeneration after treatment with EMD may be related to an increase of the mineralization markers BSP and OPN at mRNA level.
Authors: Minju Song; Bo Yu; Sol Kim; Marc Hayashi; Colby Smith; Suhjin Sohn; Euiseong Kim; James Lim; Richard G Stevenson; Reuben H Kim Journal: Dent Clin North Am Date: 2017-01
Authors: Richard J Miron; Erik Hedbom; Sabrina Ruggiero; Dieter D Bosshardt; Yufeng Zhang; Corinna Mauth; Anja C Gemperli; Tateyuki Iizuka; Daniel Buser; Anton Sculean Journal: PLoS One Date: 2011-08-15 Impact factor: 3.240
Authors: Richard J Miron; Oana M Caluseru; Vincent Guillemette; Yufeng Zhang; Anja C Gemperli; Fatiha Chandad; Anton Sculean Journal: PLoS One Date: 2013-08-12 Impact factor: 3.240