Literature DB >> 18546027

Differentiation of Aspergillus niger by random amplification of polymorphic DNA.

Kawther F Abed1.   

Abstract

The randomly amplified polymorphic DNA (RAPD) patterns of whole-cell lysates from five Aspergillus niger isolates, including one reference strain, two isolated from deep freeze, and two environmental strains from soil and plant infections, were investigated. PCR-RAPD analysis of genomic DNA was performed using eight primers (Tube-A1, Tube-A6, Tube-A17, Tube-B8, Tube-B11, Tube-B15, Tube-C5, Tube-C6). The RAPD assay discriminated between all strains. Comparison of deep freeze isolates showed identical RAPD patterns in some of the reference and environmental isolates. The data indicates that the RAPD technique is useful for fingerprinting A. niger.

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Year:  2008        PMID: 18546027     DOI: 10.1007/s10295-008-0378-x

Source DB:  PubMed          Journal:  J Ind Microbiol Biotechnol        ISSN: 1367-5435            Impact factor:   3.346


  14 in total

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Authors:  D R Soll
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Authors:  E Bart-Delabesse; J Sarfati; J P Debeaupuis; W van Leeuwen; A van Belkum; S Bretagne; J P Latge
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

3.  DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

Authors:  J G Williams; A R Kubelik; K J Livak; J A Rafalski; S V Tingey
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

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Authors:  F Symoens; J P Bouchara; S Heinemann; N Nolard
Journal:  J Hosp Infect       Date:  2000-04       Impact factor: 3.926

5.  Identification of Candida species by randomly amplified polymorphic DNA fingerprinting of colony lysates.

Authors:  P Steffan; J A Vazquez; D Boikov; C Xu; J D Sobel; R A Akins
Journal:  J Clin Microbiol       Date:  1997-08       Impact factor: 5.948

6.  Fingerprinting genomes using PCR with arbitrary primers.

Authors:  J Welsh; M McClelland
Journal:  Nucleic Acids Res       Date:  1990-12-25       Impact factor: 16.971

7.  Molecular typing of Aspergillus terreus isolates collected in Houston, Texas, and Innsbruck, Austria: evidence of great genetic diversity.

Authors:  Cornelia Lass-Flörl; Katharina Grif; Dimitrios P Kontoyiannis
Journal:  J Clin Microbiol       Date:  2007-06-20       Impact factor: 5.948

Review 8.  Molecular typing of Aspergillus species.

Authors:  M Birch; M J Anderson; D W Denning
Journal:  J Hosp Infect       Date:  1995-06       Impact factor: 3.926

9.  A molecular method for detection of Aspergillus carbonarius in coffee beans.

Authors:  Maria Helena Pelegrinelli Fungaro; Patrícia Cristina Vissotto; Danieli Sartori; Laurival Antônio Vilas-Boas; Márcia Cristina Furlaneto; Marta Hiromi Taniwaki
Journal:  Curr Microbiol       Date:  2004-08       Impact factor: 2.188

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Authors:  V Raclaský; J Trtková; L Rusková; V Buchta; R Bolehovská; M Vacková; P Hamal
Journal:  Folia Microbiol (Praha)       Date:  2006       Impact factor: 2.629

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  3 in total

1.  Diversity, molecular phylogeny and fingerprint profiles of airborne Aspergillus species using random amplified polymorphic DNA.

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Journal:  World J Microbiol Biotechnol       Date:  2016-04-27       Impact factor: 3.312

2.  Molecular characterization of Aspergillus infections in an Iranian educational hospital using RAPD-PCR method.

Authors:  Kambiz Diba; Khadijeh Makhdoomi; Hossein Mirhendi
Journal:  Iran J Basic Med Sci       Date:  2014-09       Impact factor: 2.699

3.  Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme.

Authors:  Paul Franz; Heike Betat; Mario Mörl
Journal:  BMC Microbiol       Date:  2016-03-18       Impact factor: 3.605

  3 in total

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