Literature DB >> 18540050

Structure of an N-terminally truncated selenophosphate synthetase from Aquifex aeolicus.

Eiko Matsumoto1, Shun Ichi Sekine, Ryogo Akasaka, Yumi Otta, Kazushige Katsura, Mio Inoue, Tatsuya Kaminishi, Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama.   

Abstract

Selenophosphate synthetase (SPS) catalyzes the activation of selenide with ATP to synthesize selenophosphate, the reactive selenium donor for biosyntheses of both the 21st amino acid selenocysteine and 2-selenouridine nucleotides in tRNA anticodons. The crystal structure of an N-terminally (25 residues) truncated fragment of SPS (SPS-DeltaN) from Aquifex aeolicus has been determined at 2.0 A resolution. The structure revealed SPS to be a two-domain alpha/beta protein, with domain folds that are homologous to those of PurM-superfamily proteins. In the crystal, six monomers of SPS-DeltaN form a hexamer of 204 kDa, whereas the molecular weight estimated by ultracentrifugation was approximately 63 kDa, which is comparable to the calculated weight of the dimer (68 kDa).

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Year:  2008        PMID： 18540050      PMCID： PMC2496870          DOI： 10.1107/S1744309108012074

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  32 in total

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Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

2.  X-ray crystal structure of aminoimidazole ribonucleotide synthetase (PurM), from the Escherichia coli purine biosynthetic pathway at 2.5 A resolution.

Authors:  C Li; T J Kappock; J Stubbe; T M Weaver; S E Ealick
Journal:  Structure       Date:  1999-09-15       Impact factor: 5.006

3.  Synthesis of 5-methylaminomethyl-2-selenouridine in tRNAs: 31P NMR studies show the labile selenium donor synthesized by the selD gene product contains selenium bonded to phosphorus.

Authors:  Z Veres; L Tsai; T D Scholz; M Politino; R S Balaban; T C Stadtman
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-01       Impact factor: 11.205

4.  Selenophosphate synthetase genes from lung adenocarcinoma cells: Sps1 for recycling L-selenocysteine and Sps2 for selenite assimilation.

Authors:  Takashi Tamura; Shinpei Yamamoto; Muneaki Takahata; Hiromich Sakaguchi; Hidehiko Tanaka; Thressa C Stadtman; Kenji Inagaki
Journal:  Proc Natl Acad Sci U S A       Date:  2004-11-08       Impact factor: 11.205

5.  Escherichia coli mutant SELD enzymes. The cysteine 17 residue is essential for selenophosphate formation from ATP and selenide.

Authors:  I Y Kim; Z Veres; T C Stadtman
Journal:  J Biol Chem       Date:  1992-09-25       Impact factor: 5.157

6.  Automated MAD and MIR structure solution.

Authors:  T C Terwilliger; J Berendzen
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-04

7.  Automated side-chain model building and sequence assignment by template matching.

Authors:  Thomas C Terwilliger
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-12-19

8.  Domain organization of Salmonella typhimurium formylglycinamide ribonucleotide amidotransferase revealed by X-ray crystallography.

Authors:  Ruchi Anand; Aaron A Hoskins; JoAnne Stubbe; Steven E Ealick
Journal:  Biochemistry       Date:  2004-08-17       Impact factor: 3.162

9.  Maximum-likelihood density modification.

Authors:  T C Terwilliger
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2000-08

10.  Automated main-chain model building by template matching and iterative fragment extension.

Authors:  Thomas C Terwilliger
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-12-19
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  4 in total

1.  Crystallization and preliminary X-ray diffraction analysis of selenophosphate synthetases from Trypanosoma brucei and Leishmania major.

Authors:  Lívia Maria Faim; Ivan Rosa e Silva; Marcio Vinicius Bertacine Dias; Humberto D'Muniz Pereira; José Brandao-Neto; Marco Túlio Alves da Silva; Otavio Henrique Thiemann
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2013-07-27

2.  Structural insights into the catalytic mechanism of Escherichia coli selenophosphate synthetase.

Authors:  Nicholas Noinaj; Rut Wattanasak; Duck-Yeon Lee; Jeremy L Wally; Grzegorz Piszczek; P Boon Chock; Thressa C Stadtman; Susan K Buchanan
Journal:  J Bacteriol       Date:  2011-11-11       Impact factor: 3.490

3.  Binding Stoichiometry of a Recombinant Selenophosphate Synthetase with One Synonymic Substitution E197D to a Fluorescent Nucleotide Analog of ATP, TNP-ATP.

Authors:  Y V Preobrazhenskaya; A I Stenko; M V Shvarts; V Y Lugovtsev
Journal:  J Amino Acids       Date:  2013-01-30

4.  Trypanosomatid selenophosphate synthetase structure, function and interaction with selenocysteine lyase.

Authors:  Marco Túlio Alves da Silva; Ivan Rosa E Silva; Lívia Maria Faim; Natália Karla Bellini; Murilo Leão Pereira; Ana Laura Lima; Teresa Cristina Leandro de Jesus; Fernanda Cristina Costa; Tatiana Faria Watanabe; Humberto D'Muniz Pereira; Sandro Roberto Valentini; Cleslei Fernando Zanelli; Júlio Cesar Borges; Marcio Vinicius Bertacine Dias; Júlia Pinheiro Chagas da Cunha; Bidyottam Mittra; Norma W Andrews; Otavio Henrique Thiemann
Journal:  PLoS Negl Trop Dis       Date:  2020-10-05
  4 in total

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