Literature DB >> 18538581

A rapid method for determining dynamic binding capacity of resins for the purification of proteins.

Tri Do1, Frank Ho, Bettina Heidecker, Krista Witte, Lucille Chang, Laura Lerner.   

Abstract

Biolayer interferometry is a novel method for quantifying macromolecules, such as proteins, in solution. The presence of other, non-binding molecules does not interfere with quantification, which allows one to measure the concentration of the molecule of interest in a crude mixture. Here we apply this method to determining the dynamic binding capacity of affinity resins.

Mesh:

Substances:

Year:  2008        PMID: 18538581     DOI: 10.1016/j.pep.2008.04.009

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN: 1046-5928            Impact factor:   1.650


  16 in total

Review 1.  Interferometric methods for label-free molecular interaction studies.

Authors:  Amanda Kussrow; Carolyn S Enders; Darryl J Bornhop
Journal:  Anal Chem       Date:  2011-11-07       Impact factor: 6.986

2.  Mapping and Quantitation of the Interaction between the Recombination Activating Gene Proteins RAG1 and RAG2.

Authors:  Yu-Hang Zhang; Keerthi Shetty; Marius D Surleac; Andrei J Petrescu; David G Schatz
Journal:  J Biol Chem       Date:  2015-03-05       Impact factor: 5.157

3.  Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay.

Authors:  Bo Han; Man Zhang; Peng Sun; Shangwei Hou
Journal:  J Vis Exp       Date:  2018-03-07       Impact factor: 1.355

4.  Development and Validation of Electrochemiluminescence Assays to Measure Free and Total sSLAMF7 in Human Serum in the Absence and Presence of Elotuzumab.

Authors:  Jennifer Postelnek; Robert J Neely; Michael D Robbins; Carol R Gleason; Jon E Peterson; Steven P Piccoli
Journal:  AAPS J       Date:  2016-04-26       Impact factor: 4.009

5.  Discovery of a novel inhibitor of kinesin-like protein KIFC1.

Authors:  Wei Zhang; Ling Zhai; Yimin Wang; Rebecca J Boohaker; Wenyan Lu; Vandana V Gupta; Indira Padmalayam; Robert J Bostwick; E Lucile White; Larry J Ross; Joseph Maddry; Subramaniam Ananthan; Corinne E Augelli-Szafran; Mark J Suto; Bo Xu; Rongbao Li; Yonghe Li
Journal:  Biochem J       Date:  2016-02-04       Impact factor: 3.857

6.  CMOS-integrated film bulk acoustic resonators for label-free biosensing.

Authors:  Martin Nirschl; Arto Rantala; Kari Tukkiniemi; Sanna Auer; Ann-Charlotte Hellgren; Dana Pitzer; Matthias Schreiter; Inger Vikholm-Lundin
Journal:  Sensors (Basel)       Date:  2010-04-27       Impact factor: 3.576

7.  Anti-HIV-1 activity of a new scorpion venom peptide derivative Kn2-7.

Authors:  Yaoqing Chen; Luyang Cao; Maohua Zhong; Yan Zhang; Chen Han; Qiaoli Li; Jingyi Yang; Dihan Zhou; Wei Shi; Benxia He; Fang Liu; Jie Yu; Ying Sun; Yuan Cao; Yaoming Li; Wenxin Li; Deying Guo; Zhijian Cao; Huimin Yan
Journal:  PLoS One       Date:  2012-04-19       Impact factor: 3.240

8.  Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.

Authors:  Joseph A Newman; Pavel Savitsky; Charles K Allerston; Anna H Bizard; Özgün Özer; Kata Sarlós; Ying Liu; Els Pardon; Jan Steyaert; Ian D Hickson; Opher Gileadi
Journal:  Nucleic Acids Res       Date:  2015-04-21       Impact factor: 16.971

9.  Direct Promoter Repression by BCL11A Controls the Fetal to Adult Hemoglobin Switch.

Authors:  Nan Liu; Victoria V Hargreaves; Qian Zhu; Jesse V Kurland; Jiyoung Hong; Woojin Kim; Falak Sher; Claudio Macias-Trevino; Julia M Rogers; Ryo Kurita; Yukio Nakamura; Guo-Cheng Yuan; Daniel E Bauer; Jian Xu; Martha L Bulyk; Stuart H Orkin
Journal:  Cell       Date:  2018-03-29       Impact factor: 41.582

10.  Clearance kinetics and matrix binding partners of the receptor for advanced glycation end products.

Authors:  Pavle S Milutinovic; Judson M Englert; Lauren T Crum; Neale S Mason; Lasse Ramsgaard; Jan J Enghild; Louis J Sparvero; Michael T Lotze; Tim D Oury
Journal:  PLoS One       Date:  2014-03-18       Impact factor: 3.240

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.