| Literature DB >> 18510712 |
Jun Hatakeyama1, Kenji Shimamura.
Abstract
In vitro whole-embryo culture of chick embryos, originally invented by New, has been widely used for studies of early embryogenesis. Here, a method for electroporation using the New culture and its derivatives is described, to achieve misexpression of exogenous gene in a temporally and spatially controlled manner in gastrulating chick embryos. Detailed information for the devices and procedures, and some experimental examples are presented.Entities:
Mesh:
Substances:
Year: 2008 PMID: 18510712 DOI: 10.1111/j.1440-169X.2008.01040.x
Source DB: PubMed Journal: Dev Growth Differ ISSN: 0012-1592 Impact factor: 2.053