C Bodet1, V D La, F Epifano, D Grenier. 1. Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec G1K 7P4, Canada.
Abstract
BACKGROUND AND OBJECTIVE: Periodontitis is a chronic inflammatory disease of bacterial etiology, affecting tooth-supporting tissues. The host inflammatory response to periodontopathogens, notably the high and continuous production of cytokines, is considered a major factor causing the local tissue destruction observed in periodontitis. The aim of the present study was to investigate the effect of naringenin, a major flavanone in grapefruits and tomatoes, on the lipopolysaccharide-induced pro-inflammatory cytokine production by host cells, using two different models. MATERIAL AND METHODS: The effect of naringenin was characterized using macrophages stimulated with the lipopolysaccharide of either Aggregatibacter actinomycetemcomitans or Escherichia coli and using whole blood stimulated with A. actinomycetemcomitans lipopolysaccharide, in the presence or absence of naringenin. Lipopolysaccharide-induced interleukin-1 beta, interleukin-6, interleukin-8 and tumor necrosis factor-alpha production by macrophages and whole-blood samples treated with naringenin were evaluated using an enzyme-linked immunosorbent assay. Changes in the phosphorylation states of macrophage kinases induced by A. actinomycetemcomitans lipopolysaccharide and naringenin were characterized by immunoblot screening. RESULTS: Our results clearly indicated that naringenin is a potent inhibitor of the pro-inflammatory cytokine response induced by lipopolysaccharide in both macrophages and in whole blood. Naringenin markedly inhibited the phosphorylation on serines 63 and 73 of Jun proto-oncogene-encoded AP-1 transcription factor in lipopolysaccharide-stimulated macrophages. CONCLUSION: The results from the present study suggest that naringenin holds promise as a therapeutic agent for treating inflammatory diseases such as periodontitis.
BACKGROUND AND OBJECTIVE:Periodontitis is a chronic inflammatory disease of bacterial etiology, affecting tooth-supporting tissues. The host inflammatory response to periodontopathogens, notably the high and continuous production of cytokines, is considered a major factor causing the local tissue destruction observed in periodontitis. The aim of the present study was to investigate the effect of naringenin, a major flavanone in grapefruits and tomatoes, on the lipopolysaccharide-induced pro-inflammatory cytokine production by host cells, using two different models. MATERIAL AND METHODS: The effect of naringenin was characterized using macrophages stimulated with the lipopolysaccharide of either Aggregatibacter actinomycetemcomitans or Escherichia coli and using whole blood stimulated with A. actinomycetemcomitanslipopolysaccharide, in the presence or absence of naringenin. Lipopolysaccharide-induced interleukin-1 beta, interleukin-6, interleukin-8 and tumor necrosis factor-alpha production by macrophages and whole-blood samples treated with naringenin were evaluated using an enzyme-linked immunosorbent assay. Changes in the phosphorylation states of macrophage kinases induced by A. actinomycetemcomitanslipopolysaccharide and naringenin were characterized by immunoblot screening. RESULTS: Our results clearly indicated that naringenin is a potent inhibitor of the pro-inflammatory cytokine response induced by lipopolysaccharide in both macrophages and in whole blood. Naringenin markedly inhibited the phosphorylation on serines 63 and 73 of Jun proto-oncogene-encoded AP-1 transcription factor in lipopolysaccharide-stimulated macrophages. CONCLUSION: The results from the present study suggest that naringenin holds promise as a therapeutic agent for treating inflammatory diseases such as periodontitis.
Authors: Adriana G Guimarães; Silvana V F Gomes; Valéria R S Moraes; Paulo C L Nogueira; Antônio G Ferreira; Arie F Blank; Alan D C Santos; Monalisa D Viana; Geraldo H Silva; Lucindo J Quintans Júnior Journal: J Nat Med Date: 2011-10-22 Impact factor: 2.343
Authors: Michael Naesby; Søren Vs Nielsen; Curt Af Nielsen; Trine Green; Thomas O Tange; Ernesto Simón; Philipp Knechtle; Anders Hansson; Markus S Schwab; Olca Titiz; Christophe Folly; Roberto E Archila; Milena Maver; Stephan van Sint Fiet; Thiamo Boussemghoune; Michael Janes; A S Sathish Kumar; Shailendra P Sonkar; Partha P Mitra; V Ajai Kumar Benjamin; Nimitha Korrapati; Inala Suman; Esben H Hansen; Tanja Thybo; Neil Goldsmith; Alexandra Santana Sorensen Journal: Microb Cell Fact Date: 2009-08-13 Impact factor: 5.328