Pengde Kang1, Bin Shen, Jing Yang, Fuxing Pei. 1. Orthopaedic Department, West China Hospital, Sichuan University, Chengdu (610041), People's Republic of China.
Abstract
INTRODUCTION: To test the hypothesis that the platelet microparticle (PMP) and endothelial microparticle (EMP) may contribute to the hypercoagulability associated with microvascular thrombosis in patients with nontraumatc osteonecrosis of the femoral head (ONFH). MATERIALS AND METHODS: The study comprised 46 patients who had been diagnosed with ONFH and 20 control subjects. The plasma was ultracentrifuged, and then PMPs and EMPs were examined by the flow cytometry. The thrombotic and fibrinolytic disorders were investigated. RESULTS: The numbers of PMPs expressing P-selectin and CD42a and EMPs expressing E-selectin and CD31 in the ONFH patients were significantly higher than those in the controls (P<0.001). The number of MPs was correlated with the level of the serum C-reactive protein (CRP) (r=0.661, P<0.001), but there was a poor correlation between the MPs counts and the risk factors for ONFH (P>0.05). The mean levels PAI-1, F1+2, and TAT were higher in the patients with ONFH than in the controls (P<0.05). CONCLUSIONS: The elevated numbers of PMPs and EMPs may contribute to hypercoagulability in the ONFH patients. This may provide important pathophysiological insights into the hypercoagulability associated with nontraumatic ONFH and have implications for pharmacological prevention and treatment of ONFH.
INTRODUCTION: To test the hypothesis that the platelet microparticle (PMP) and endothelial microparticle (EMP) may contribute to the hypercoagulability associated with microvascular thrombosis in patients with nontraumatc osteonecrosis of the femoral head (ONFH). MATERIALS AND METHODS: The study comprised 46 patients who had been diagnosed with ONFH and 20 control subjects. The plasma was ultracentrifuged, and then PMPs and EMPs were examined by the flow cytometry. The thrombotic and fibrinolytic disorders were investigated. RESULTS: The numbers of PMPs expressing P-selectin and CD42a and EMPs expressing E-selectin and CD31 in the ONFH patients were significantly higher than those in the controls (P<0.001). The number of MPs was correlated with the level of the serum C-reactive protein (CRP) (r=0.661, P<0.001), but there was a poor correlation between the MPs counts and the risk factors for ONFH (P>0.05). The mean levels PAI-1, F1+2, and TAT were higher in the patients with ONFH than in the controls (P<0.05). CONCLUSIONS: The elevated numbers of PMPs and EMPs may contribute to hypercoagulability in the ONFH patients. This may provide important pathophysiological insights into the hypercoagulability associated with nontraumatic ONFH and have implications for pharmacological prevention and treatment of ONFH.
Authors: Keely L Buesing; John C Densmore; Sushma Kaul; Kirkwood A Pritchard; Jason A Jarzembowski; David M Gourlay; Keith T Oldham Journal: J Surg Res Date: 2010-06-09 Impact factor: 2.192
Authors: Angelo Nascimbene; Ruben Hernandez; Joggy K George; Anita Parker; Angela L Bergeron; Subhashree Pradhan; K Vinod Vijayan; Andrew Civitello; Leo Simpson; Maria Nawrot; Vei-Vei Lee; Hari R Mallidi; Reynolds M Delgado; Jing Fei Dong; O H Frazier Journal: J Heart Lung Transplant Date: 2014-01-19 Impact factor: 10.247
Authors: Sing Chung Li; Ching Yu Lin; Tzong Fu Kuo; Yun Ho Lin; Chia Chun Chen; Way Neng Lin; Wing P Chan Journal: Proteome Sci Date: 2010-09-14 Impact factor: 2.480
Authors: Abdelhafeez H Abdelhafeez; Paul M Jeziorczak; Terry R Schaid; Susan L Hoefs; Sushma Kaul; Rahul Nanchal; Elizabeth R Jacobs; John C Densmore Journal: J Extracell Vesicles Date: 2014-02-27