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Literature DB >> 1848754

An acetylation method for the quantification of membrane lipids, including phospholipids, polyphosphoinositides and cholesterol.

Abstract

A method for the quantification of membrane lipids by acetylation with [3H]acetic anhydride is described. A standard lipid, labelled with 14C or 32P, is added to the sample and is simultaneously acetylated. The 3H/14C or 3H/32P ratios obtained in the acetylated lipids are proportional to the initial lipid concentration. The method has been used to quantify cholesterol, phosphatidylethanolamine, phosphatidylinositol, sphingomyelin, phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in amounts between 2 and 10 nmol.

Entities: Chemical

Mesh：

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Year: 1991 PMID： 1848754 PMCID： PMC1150147 DOI： 10.1042/bj2740375

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

11 in total

9. Ectocytosis caused by sublytic autologous complement attack on human neutrophils. The sorting of endogenous plasma-membrane proteins and lipids into shed vesicles.

Authors: J M Stein; J P Luzio
Journal: Biochem J Date: 1991-03-01 Impact factor: 3.857

10. Remodeling of granulocyte membrane fatty acids during phagocytosis.

Authors: J E Smolen; S B Shohet
Journal: J Clin Invest Date: 1974-03 Impact factor: 14.808

1 in total

1. Ectocytosis caused by sublytic autologous complement attack on human neutrophils. The sorting of endogenous plasma-membrane proteins and lipids into shed vesicles.

Authors: J M Stein; J P Luzio
Journal: Biochem J Date: 1991-03-01 Impact factor: 3.857

1 in total

An acetylation method for the quantification of membrane lipids, including phospholipids, polyphosphoinositides and cholesterol.

1. A rapid method of total lipid extraction and purification.

2. Determination of cholesterol using o-phthalaldehyde.

3. Phosphorus assay in column chromatography.

4. A simple method for the isolation and purification of total lipides from animal tissues.

5. A calcium-activated polyphosphoinositide phosphodiesterase in the plasma membrane of human and rabbit erythrocytes.

6. Regional distribution of polyphosphoinositides in rat brain.

7. Rapid decrease of phosphatidylinositol 4,5-bisphosphate in thrombin-stimulated platelets.

8. A new micromethod for the colorimetric determination of inorganic phosphate.

9. Ectocytosis caused by sublytic autologous complement attack on human neutrophils. The sorting of endogenous plasma-membrane proteins and lipids into shed vesicles.

10. Remodeling of granulocyte membrane fatty acids during phagocytosis.

1. Ectocytosis caused by sublytic autologous complement attack on human neutrophils. The sorting of endogenous plasma-membrane proteins and lipids into shed vesicles.