Angel Josabad Alonso-Castro1, Luis A Salazar-Olivo. 1. Instituto Potosino de Investigación Científica y Tecnológica, División de Biología Molecular, Camino a la Presa San José 2055, Lomas 4a secc., 78216 San Luis Potosí, SLP, Mexico.
Abstract
ETHNOPHARMACOLOGICAL IMPORTANCE: Guazuma ulmifolia Lam (Sterculiaceae) is a plant extensively used in México for the empirical treatment of type 2 diabetes. AIM OF THE STUDY: To investigate the anti-diabetic mechanisms of Guazuma ulmifolia. MATERIALS AND METHODS: Non-toxic concentrations of Guazuma ulmifolia aqueous extracts (GAE) were assayed on adipogenesis and 2-NBDglucose uptake in the murine 3T3-F442A preadipose cell line. RESULTS: GAE added to adipogenic medium (AM) did not affect adipogenesis at any of the tested concentrations (1-70 microg/ml), whereas in AM lacking insulin GAE 70 microg/ml induced triglyceride accumulation by 23%. On the other hand, GAE 70 microg/ml stimulated 2-NBDG uptake by 40% in insulin-sensitive 3T3-F442A adipocytes and by 24% in insulin-resistant adipocytes, with respect to the incorporation showed by insulin-sensitive adipocytes stimulated with the hormone. CONCLUSION: Guazuma ulmifolia exerts its anti-diabetic effects by stimulating glucose uptake in both insulin-sensitive and insulin-resistant adipocytes without inducing adipogenesis.
ETHNOPHARMACOLOGICAL IMPORTANCE: Guazuma ulmifolia Lam (Sterculiaceae) is a plant extensively used in México for the empirical treatment of type 2 diabetes. AIM OF THE STUDY: To investigate the anti-diabetic mechanisms of Guazuma ulmifolia. MATERIALS AND METHODS: Non-toxic concentrations of Guazuma ulmifolia aqueous extracts (GAE) were assayed on adipogenesis and 2-NBDglucose uptake in the murine 3T3-F442A preadipose cell line. RESULTS:GAE added to adipogenic medium (AM) did not affect adipogenesis at any of the tested concentrations (1-70 microg/ml), whereas in AM lacking insulin GAE 70 microg/ml induced triglyceride accumulation by 23%. On the other hand, GAE 70 microg/ml stimulated 2-NBDG uptake by 40% in insulin-sensitive 3T3-F442A adipocytes and by 24% in insulin-resistant adipocytes, with respect to the incorporation showed by insulin-sensitive adipocytes stimulated with the hormone. CONCLUSION:Guazuma ulmifolia exerts its anti-diabetic effects by stimulating glucose uptake in both insulin-sensitive and insulin-resistant adipocytes without inducing adipogenesis.
Authors: Adam De Jesus; Farnaz Keyhani-Nejad; Carolina M Pusec; Lauren Goodman; Justin A Geier; Joshua S Stoolman; Paulina J Stanczyk; Tivoli Nguyen; Kai Xu; Krishna V Suresh; Yihan Chen; Arianne E Rodriguez; Jason S Shapiro; Hsiang-Chun Chang; Chunlei Chen; Kriti P Shah; Issam Ben-Sahra; Brian T Layden; Navdeep S Chandel; Samuel E Weinberg; Hossein Ardehali Journal: Mol Cell Date: 2022-03-18 Impact factor: 17.970
Authors: Gisely C Lopes; Renata Longhini; Paulo Victor P Dos Santos; Adriano A S Araújo; Marcos Luciano Bruschi; João Carlos P de Mello Journal: Int J Anal Chem Date: 2012-01-12 Impact factor: 1.885
Authors: Ana Carolina Mazei Silva; Maísa Pavani Dos Santos; Suélem Aparecida de França; Virginia Claudia da Silva; Luiz Everson da Silva; Uir Santana de Figueiredo; Evandro Luiz Dall'Oglio; Paulo Teixeira de Sousa Júnior; Carbene França Lopes; Amanda Martins Baviera; Nair Honda Kawashita Journal: J Intercult Ethnopharmacol Date: 2014-11-28
Authors: José Tarcísio de Giffoni de Carvalho; Débora da Silva Baldivia; Daniel Ferreira Leite; Laura Costa Alves de Araújo; Priscilla Pereira de Toledo Espindola; Katia Avila Antunes; Paola Santos Rocha; Kely de Picoli Souza; Edson Lucas Dos Santos Journal: Oxid Med Cell Longev Date: 2019-08-25 Impact factor: 6.543